{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T11:04:49Z","timestamp":1777460689313,"version":"3.51.4"},"reference-count":17,"publisher":"Wiley","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["CRSII3_125447"],"award-info":[{"award-number":["CRSII3_125447"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["CP-IP 228933-2"],"award-info":[{"award-number":["CP-IP 228933-2"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011263","name":"FP7-NMP","doi-asserted-by":"crossref","award":["CRSII3_125447"],"award-info":[{"award-number":["CRSII3_125447"]}],"id":[{"id":"10.13039\/100011263","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100011263","name":"FP7-NMP","doi-asserted-by":"crossref","award":["CP-IP 228933-2"],"award-info":[{"award-number":["CP-IP 228933-2"]}],"id":[{"id":"10.13039\/100011263","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Computational and Mathematical Methods in Medicine"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>Deep tissue imaging has become state of the art in biology, but now the problem is to quantify spatial information in a global, organ-wide context. Although access to the raw data is no longer a limitation, the computational tools to extract biologically useful information out of these large data sets is still catching up. In many cases, to understand the mechanism behind a biological process, where molecules or cells interact with each other, it is mandatory to know their mutual positions. We illustrate this principle here with the immune system. Although the general functions of lymph nodes as immune sentinels are well described, many cellular and molecular details governing the interactions of lymphocytes and dendritic cells remain unclear to date and prevent an in-depth mechanistic understanding of the immune system. We imaged<jats:italic>ex vivo<\/jats:italic>lymph nodes isolated from both wild-type and transgenic mice lacking key factors for dendritic cell positioning and used software written in MATLAB to determine the spatial distances between the dendritic cells and the internal high endothelial vascular network. This allowed us to quantify the spatial localization of the dendritic cells in the lymph node, which is a critical parameter determining the effectiveness of an adaptive immune response.<\/jats:p>","DOI":"10.1155\/2012\/128431","type":"journal-article","created":{"date-parts":[[2012,9,16]],"date-time":"2012-09-16T21:01:26Z","timestamp":1347829286000},"page":"1-8","source":"Crossref","is-referenced-by-count":16,"title":["Quantitative Measurements in 3-Dimensional Datasets of Mouse Lymph Nodes Resolve Organ-Wide Functional Dependencies"],"prefix":"10.1155","volume":"2012","author":[{"given":"J\u00fcrgen","family":"Mayer","sequence":"first","affiliation":[{"name":"EMBL\/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG) and UPF, Dr. Aiguader 88, 08003 Barcelona, Spain"}]},{"given":"Jim","family":"Swoger","sequence":"additional","affiliation":[{"name":"EMBL\/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG) and UPF, Dr. Aiguader 88, 08003 Barcelona, Spain"}]},{"given":"Aleksandra J.","family":"Ozga","sequence":"additional","affiliation":[{"name":"Theodor Kocher Institut, Universit\u00e4t Bern, Freiestrasse 1, 3012 Bern, Switzerland"}]},{"given":"Jens V.","family":"Stein","sequence":"additional","affiliation":[{"name":"Theodor Kocher Institut, Universit\u00e4t Bern, Freiestrasse 1, 3012 Bern, Switzerland"}]},{"given":"James","family":"Sharpe","sequence":"additional","affiliation":[{"name":"EMBL\/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG) and UPF, Dr. Aiguader 88, 08003 Barcelona, Spain"}]}],"member":"311","reference":[{"key":"1","year":"2006"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1100035"},{"key":"3","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1146\/annurev.bioeng.6.040803.140210","volume":"6","year":"2004","journal-title":"Annual Review of Biomedical Engineering"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1734"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.15.8206"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1126\/science.1127344"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth929"},{"issue":"11","key":"18","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1038\/nri1222","volume":"3","year":"2003","journal-title":"Nature Reviews Immunology"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2012.02.007"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth818"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1126\/science.1068206"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1109\/TIP.2002.806250"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot5639"},{"issue":"2","key":"24","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.4049\/jimmunol.172.2.1146","volume":"172","year":"2004","journal-title":"Journal of Immunology"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.3.451"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1006\/ciun.1993.1029"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1021\/ci034150f"}],"container-title":["Computational and Mathematical Methods in Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/cmmm\/2012\/128431.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cmmm\/2012\/128431.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cmmm\/2012\/128431.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T05:26:09Z","timestamp":1588829169000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/cmmm\/2012\/128431\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":17,"alternative-id":["128431","128431"],"URL":"https:\/\/doi.org\/10.1155\/2012\/128431","relation":{},"ISSN":["1748-670X","1748-6718"],"issn-type":[{"value":"1748-670X","type":"print"},{"value":"1748-6718","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}