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Apolipoprotein E (ApoE) is a 34-kDa circulating glycoprotein, secreted by the liver and macrophages with pleiotropic antiatherogenic functions and hence a candidate to treat hypercholesterolaemia and atherosclerosis. Here, we describe atheroprotective properties of ApoE, though also potential proatherogenic actions, and the prevalence of dysfunctional isoforms, outline conventional gene transfer strategies, and then focus on gene correction therapeutics that can repair defective<jats:italic>APOE<\/jats:italic>alleles. In particular, we discuss the possibility and potential benefit of applying in combination two technical advances to repair aberrant<jats:italic>APOE<\/jats:italic>genes: (i) an engineered endonuclease to introduce a double-strand break (DSB) in exon 4, which contains the common, but dysfunctional,<jats:italic>\u03b5<\/jats:italic>2 and<jats:italic>\u03b5<\/jats:italic>4 alleles; (ii) an efficient and selectable template for homologous recombination (HR) repair, namely, an adeno-associated viral (AAV) vector, which harbours wild-type<jats:italic>APOE<\/jats:italic>sequence. This technology is applicable ex vivo, for example to target haematopoietic or induced pluripotent stem cells, and also for in vivo hepatic gene targeting. It is to be hoped that such emerging technology will eventually translate to patient therapy to reduce CVD risk.<\/jats:p>","DOI":"10.1155\/2012\/148796","type":"journal-article","created":{"date-parts":[[2012,5,8]],"date-time":"2012-05-08T03:34:26Z","timestamp":1336448066000},"page":"1-16","source":"Crossref","is-referenced-by-count":11,"title":["Targeted In Situ Gene Correction of Dysfunctional<i>APOE<\/i>Alleles to Produce Atheroprotective Plasma ApoE3 Protein"],"prefix":"10.1155","volume":"2012","author":[{"given":"Ioannis","family":"Papaioannou","sequence":"first","affiliation":[{"name":"Division of Medicine, UCL Medical School, Royal Free Campus, Rowland Hill Street, London NW3 2PF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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