{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:28:17Z","timestamp":1788870497207,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","license":[{"start":{"date-parts":[[2012,12,4]],"date-time":"2012-12-04T00:00:00Z","timestamp":1354579200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["32-31405.91"],"award-info":[{"award-number":["32-31405.91"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Florindon Foundation","award":["32-31405.91"],"award-info":[{"award-number":["32-31405.91"]}]},{"DOI":"10.13039\/501100004794","name":"Centre national de la recherche scientifique","doi-asserted-by":"publisher","award":["32-31405.91"],"award-info":[{"award-number":["32-31405.91"]}],"id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["32-31405.91"],"award-info":[{"award-number":["32-31405.91"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["32-31405.91"],"award-info":[{"award-number":["32-31405.91"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Le Studium Orl\u00e9ans","award":["32-31405.91"],"award-info":[{"award-number":["32-31405.91"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Allergy"],"published-print":{"date-parts":[[2012,12,4]]},"abstract":"<jats:p>\n                    Leukocyte adhesion molecules are involved in cell recruitment in an allergic airway response and therefore provide a target for pharmaceutical intervention. Neutrophil inhibitory factor (NIF), derived from canine hookworm (\n                    <jats:italic>Ancylostoma caninum<\/jats:italic>\n                    ), binds selectively and competes with the A-domain of CD11b for binding to ICAM-1. The effect of recombinant NIF was investigated. Intranasal administration of rNIF reduced pulmonary eosinophilic infiltration, goblet cell hyperplasia, and Th\n                    <jats:sub>2<\/jats:sub>\n                    cytokine production in OVA-sensitized mice.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , transendothelial migration of human blood eosinophils across IL-4-activated umbilical vein endothelial cell (HUVEC) monolayers was inhibited by rNIF (IC\n                    <jats:sub>50<\/jats:sub>\n                    :\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                      <mml:mrow>\n                        <mml:mn>4.6<\/mml:mn>\n                        <mml:mo>\u00b1<\/mml:mo>\n                        <mml:mn>2.6<\/mml:mn>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    \u2009nM; mean \u00b1 SEM), but not across TNF or IL-1-activated HUVEC monolayers. Treatment of eosinophils with rNIF together with mAb 60.1 directed against CD11b or mAb 107 directed against the metal ion-dependent adhesion site (MIDAS) of the CD11b A-domain resulted in no further inhibition of transendothelial migration suggesting shared functional epitopes. In contrast, rNIF increased the inhibitory effect of blocking mAbs against CD18, CD11a, and VLA-4. Together, we show that rNIF, a selective antagonist of the A-domain of CD11b, has a prominent inhibitory effect on eosinophil transendothelial migration\n                    <jats:italic>in vitro<\/jats:italic>\n                    , which is congruent to the\n                    <jats:italic>in vivo<\/jats:italic>\n                    inhibition of OVA-induced allergic lung inflammation.\n                  <\/jats:p>","DOI":"10.1155\/2012\/245909","type":"journal-article","created":{"date-parts":[[2012,12,4]],"date-time":"2012-12-04T17:10:03Z","timestamp":1354641003000},"page":"1-10","source":"Crossref","is-referenced-by-count":6,"title":["Neutrophil Inhibitory Factor Selectively Inhibits the Endothelium-Driven Transmigration of Eosinophils\n                    <i>In Vitro<\/i>\n                    and Airway Eosinophilia in OVA-Induced Allergic Lung Inflammation"],"prefix":"10.1155","volume":"2012","author":[{"given":"Silvia","family":"Schnyder-Candrian","sequence":"first","affiliation":[{"name":"UMR7355, CNRS, Orleans, France"},{"name":"Experimental and Molecular Immunology and Neurogenetics, University of Orleans, Orleans, France"},{"name":"Institute for Clinical and Biomedical Research Thurgau, 9548 Matzingen, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Maillet","sequence":"additional","affiliation":[{"name":"UMR7355, CNRS, Orleans, France"},{"name":"Experimental and Molecular Immunology and Neurogenetics, University of Orleans, Orleans, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Le Bert","sequence":"additional","affiliation":[{"name":"UMR7355, CNRS, Orleans, France"},{"name":"Experimental and Molecular Immunology and Neurogenetics, University of Orleans, Orleans, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea","family":"Brault","sequence":"additional","affiliation":[{"name":"UMR7355, CNRS, Orleans, France"},{"name":"Experimental and Molecular Immunology and Neurogenetics, University of Orleans, Orleans, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muazzam","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Institute of Infectious Disease and Molecular Medicine (IIDMM), University of Cape Town, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Ryffel","sequence":"additional","affiliation":[{"name":"UMR7355, CNRS, Orleans, France"},{"name":"Experimental and Molecular Immunology and Neurogenetics, University of Orleans, Orleans, France"},{"name":"Institute of Infectious Disease and Molecular Medicine (IIDMM), University of Cape Town, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Schnyder","sequence":"additional","affiliation":[{"name":"UMR7355, CNRS, Orleans, France"},{"name":"Experimental and Molecular Immunology and Neurogenetics, University of Orleans, Orleans, France"},{"name":"Institute for Clinical and Biomedical Research Thurgau, 9548 Matzingen, Switzerland"},{"name":"UAS, HES-SO, Route de Rawyl 47, 1950 Sion, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9","family":"Moser","sequence":"additional","affiliation":[{"name":"Institute for Clinical and Biomedical Research Thurgau, 9548 Matzingen, Switzerland"},{"name":"IBR Inc., Institute for Biopharmaceutical Research, Lauchefeld, 9548 Matzingen, 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