{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:57:41Z","timestamp":1758635861822,"version":"3.37.3"},"reference-count":31,"publisher":"Wiley","license":[{"start":{"date-parts":[[2012,6,7]],"date-time":"2012-06-07T00:00:00Z","timestamp":1339027200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK37034","DK073336","W81XWH-09-1-0484","P30 CA138313"],"award-info":[{"award-number":["DK37034","DK073336","W81XWH-09-1-0484","P30 CA138313"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK37034","DK073336","W81XWH-09-1-0484","P30 CA138313"],"award-info":[{"award-number":["DK37034","DK073336","W81XWH-09-1-0484","P30 CA138313"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["DK37034","DK073336","W81XWH-09-1-0484","P30 CA138313"],"award-info":[{"award-number":["DK37034","DK073336","W81XWH-09-1-0484","P30 CA138313"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Cancer Center","award":["DK37034","DK073336","W81XWH-09-1-0484","P30 CA138313"],"award-info":[{"award-number":["DK37034","DK073336","W81XWH-09-1-0484","P30 CA138313"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["HPB Surgery"],"published-print":{"date-parts":[[2012,6,7]]},"abstract":"<jats:p>Patients that survive hemorrhage and resuscitation (H\/R) may develop a systemic inflammatory response syndrome (SIRS) that leads to dysfunction of vital organs (multiple organ dysfunction syndrome, MODS). SIRS and MODS may involve mitochondrial dysfunction. Under pentobarbital anesthesia, C57BL6 mice were hemorrhaged to 30\u2009mm\u2009Hg for 3\u2009h and then resuscitated with shed blood plus half the volume of lactated Ringer\u2019s solution containing minocycline, tetracycline (both 10\u2009mg\/kg body weight) or vehicle. Serum alanine aminotransferase (ALT), necrosis, apoptosis and oxidative stress were assessed 6 h after resuscitation. Mitochondrial polarization was assessed by intravital microscopy. After H\/R with vehicle or tetracycline, ALT increased to 4538\u2009U\/L and 3999\u2009U\/L, respectively, which minocycline decreased to 1763\u2009U\/L (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.01<\/mml:mn><\/mml:math>). Necrosis and TUNEL also decreased from 24.5% and 17.7 cells\/field, respectively, after vehicle to 8.3% and 8.7 cells\/field after minocycline. Tetracycline failed to decrease necrosis (23.3%) but decreased apoptosis to 9 cells\/field (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>). Minocycline and tetracycline also decreased caspase-3 activity in liver homogenates. Minocycline but not tetracycline decreased lipid peroxidation after resuscitation by 70% (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>). Intravital microscopy showed that minocycline preserved mitochondrial polarization after H\/R (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>). In conclusion, minocycline decreases liver injury and oxidative stress after H\/R by preventing mitochondrial dysfunction.<\/jats:p>","DOI":"10.1155\/2012\/259512","type":"journal-article","created":{"date-parts":[[2012,6,7]],"date-time":"2012-06-07T21:01:09Z","timestamp":1339102869000},"page":"1-9","source":"Crossref","is-referenced-by-count":10,"title":["Minocycline Decreases Liver Injury after Hemorrhagic Shock and Resuscitation in Mice"],"prefix":"10.1155","volume":"2012","author":[{"given":"Christoph","family":"Czerny","sequence":"first","affiliation":[{"name":"Center for Cell Death, Injury & Regeneration, Department of Pharmaceutical & Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425, USA"},{"name":"Department of Trauma Surgery, J. W. Goethe University, 60590 Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andaleb","family":"Kholmukhamedov","sequence":"additional","affiliation":[{"name":"Center for Cell Death, Injury & Regeneration, Department of Pharmaceutical & Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom P.","family":"Theruvath","sequence":"additional","affiliation":[{"name":"Center for Cell Death, Injury & Regeneration, Department of Pharmaceutical & Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo N.","family":"Maldonado","sequence":"additional","affiliation":[{"name":"Center for Cell Death, Injury & Regeneration, Department of Pharmaceutical & Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Venkat K.","family":"Ramshesh","sequence":"additional","affiliation":[{"name":"Center for Cell Death, Injury & Regeneration, Department of Pharmaceutical & Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Lehnert","sequence":"additional","affiliation":[{"name":"Department of Trauma Surgery, J. W. Goethe University, 60590 Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingo","family":"Marzi","sequence":"additional","affiliation":[{"name":"Department of Trauma Surgery, J. W. Goethe University, 60590 Frankfurt am Main, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi","family":"Zhong","sequence":"additional","affiliation":[{"name":"Center for Cell Death, Injury & Regeneration, Department of Pharmaceutical & Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J.","family":"Lemasters","sequence":"additional","affiliation":[{"name":"Center for Cell Death, Injury & Regeneration, Department of Pharmaceutical & Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425, USA"},{"name":"Department of Biochemistry & Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1097\/MCC.0b013e3283196522"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22187"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2009.06.009"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.90287.2008"},{"issue":"3","key":"5","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1097\/00005373-199809000-00022","volume":"45","year":"1998","journal-title":"The Journal of Trauma"},{"issue":"4","key":"6","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1007\/s00395-003-0415-x","volume":"98","year":"2003","journal-title":"Basic Research in Cardiology"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21912"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706079"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra022366"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00050.2004"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2202-10-126"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313629200"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1038\/417074a"},{"key":"14","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.bbr.2008.09.040","volume":"196","year":"2009","journal-title":"Behavioural Brain Research"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00480.2005"},{"issue":"2","key":"16","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1097\/00024382-200013020-00008","volume":"13","year":"2000","journal-title":"Shock"},{"issue":"2","key":"17","first-page":"H791","volume":"279","year":"2000","journal-title":"American Journal of Physiology"},{"issue":"4","key":"18","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1097\/00024382-200304000-00009","volume":"19","year":"2003","journal-title":"Shock"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2006.10.171"},{"key":"20","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1097\/SHK.0b013e31815dd623","volume":"30","year":"2008","journal-title":"Shock"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2001.22002"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(03)01209-5"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.16.11814"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(03)01066-7"},{"issue":"1","key":"25","doi-asserted-by":"crossref","first-page":"G1","DOI":"10.1152\/ajpcell.1999.276.1.C1","volume":"276","year":"1999","journal-title":"The American journal of physiology"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)00618-1"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/0162-0134(88)80049-7"},{"issue":"12","key":"28","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.1002\/jps.2600651209","volume":"65","year":"1976","journal-title":"Journal of Pharmaceutical Sciences"},{"issue":"1","key":"29","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1089\/109629600321263","volume":"1","year":"2000","journal-title":"Surgical infections"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1089\/sur.2006.7.327"},{"issue":"3","key":"31","first-page":"176","volume":"86","year":"2001","journal-title":"International Surgery"}],"container-title":["HPB Surgery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/archive\/2012\/259512.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/archive\/2012\/259512.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/archive\/2012\/259512.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,17]],"date-time":"2022-01-17T01:52:45Z","timestamp":1642384365000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/hpb\/2012\/259512\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,7]]},"references-count":31,"alternative-id":["259512","259512"],"URL":"https:\/\/doi.org\/10.1155\/2012\/259512","relation":{},"ISSN":["0894-8569","1607-8462"],"issn-type":[{"type":"print","value":"0894-8569"},{"type":"electronic","value":"1607-8462"}],"subject":[],"published":{"date-parts":[[2012,6,7]]}}}