{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T14:20:34Z","timestamp":1758637234080,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003201","name":"Agro-Biotechnology Institute","doi-asserted-by":"publisher","award":["06-05-ABI-AB002"],"award-info":[{"award-number":["06-05-ABI-AB002"]}],"id":[{"id":"10.13039\/501100003201","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","award":["06-05-ABI-AB002"],"award-info":[{"award-number":["06-05-ABI-AB002"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biomedicine and Biotechnology"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>Attenuated<jats:italic>Salmonella<\/jats:italic>has been used as a carrier for DNA vaccine. However,<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>studies on the bacteria following transfection of plasmid DNA were poorly studied. In this paper, eukaryotic expression plasmids encoding avian influenza virus (AIV) subtype H5N1 genes, pcDNA3.1\/HA, NA, and NP, were transfected into an attenuated<jats:italic>Salmonella enteric typhimurium<\/jats:italic>SV4089.<jats:italic>In vitro<\/jats:italic>stability of the transfected plasmids into<jats:italic>Salmonella<\/jats:italic>were over 90% after 100 generations. The attenuated<jats:italic>Salmonella<\/jats:italic>were able to invade MCF-7 (1.2%) and MCF-10A (0.5%) human breast cancer cells. Newly hatched specific-pathogen-free (SPF) chicks were inoculated once by oral gavage with 10<jats:sup>9<\/jats:sup>colony-forming unit (CFU) of the attenuated<jats:italic>Salmonella<\/jats:italic>. No abnormal clinical signs or deaths were recorded after inoculation. Viable bacteria were detected 3 days after inoculation by plating from spleen, liver, and cecum. Fluorescent<jats:italic>in situ<\/jats:italic>hybridization (FISH) and polymerase chain reaction (PCR) were carried out for confirmation.<jats:italic>Salmonella<\/jats:italic>was not detected in blood cultures although serum antibody immune responses to<jats:italic>Salmonella<\/jats:italic>O antiserum group D1 factor 1, 9, and 12 antigens were observed in all the inoculated chickens after 7 days up to 35 days. Our results showed that live attenuated<jats:italic>S. typhimurium<\/jats:italic>SV4089 harboring pcDNA3.1\/HA, NA, and NP may provide a unique alternative as a carrier for DNA oral vaccine in chickens.<\/jats:p>","DOI":"10.1155\/2012\/264986","type":"journal-article","created":{"date-parts":[[2012,6,5]],"date-time":"2012-06-05T02:55:46Z","timestamp":1338864946000},"page":"1-8","source":"Crossref","is-referenced-by-count":7,"title":["Attenuated<i>Salmonella typhimurium<\/i>SV4089 as a Potential Carrier of Oral DNA Vaccine in Chickens"],"prefix":"10.1155","volume":"2012","author":[{"given":"Seyed Davoud","family":"Jazayeri","sequence":"first","affiliation":[{"name":"Institute of Bioscience, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aini","family":"Ideris","sequence":"additional","affiliation":[{"name":"Institute of Bioscience, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia"},{"name":"Faculty of Veterinary Medicine, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zunita","family":"Zakaria","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdul Rahman","family":"Omar","sequence":"additional","affiliation":[{"name":"Institute of Bioscience, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia"},{"name":"Faculty of Veterinary Medicine, University Putra Malaysia, 43400 Serdang, Selangor, 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