{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T02:17:00Z","timestamp":1718331420438},"reference-count":20,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Case Reports in Dermatological Medicine"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>We report two occupationally acquired cases of American cutaneous leishmaniasis (ACL): one accidental laboratory autoinoculation by contaminated needlestick while handling an ACL lesion sample, and one acquired during field studies on bird biology. Polymerase chain reaction (PCR) assays of patient lesions were positive for<jats:italic>Leishmania<\/jats:italic>, subgenus<jats:italic>Viannia<\/jats:italic>. One isolate was obtained by culture (from patient 2 biopsy samples) and characterized as<jats:italic>Leishmania<\/jats:italic>(<jats:italic>Viannia<\/jats:italic>)<jats:italic>naiffi<\/jats:italic>through an indirect immunofluorescence assay (IFA) with species-specific monoclonal antibodies (mAbs) and by multilocus enzyme electrophoresis (MLEE). Patients were successfully treated with N-methyl-glucamine. These two cases highlight the potential risks of laboratory and field work and the need to comply with strict biosafety procedures in daily routines. The swab collection method, coupled with PCR detection, has greatly improved ACL laboratory diagnosis.<\/jats:p>","DOI":"10.1155\/2012\/279517","type":"journal-article","created":{"date-parts":[[2012,11,28]],"date-time":"2012-11-28T21:25:02Z","timestamp":1354137902000},"page":"1-4","source":"Crossref","is-referenced-by-count":4,"title":["Occupationally Acquired American Cutaneous Leishmaniasis"],"prefix":"10.1155","volume":"2012","author":[{"given":"Maria Edileuza","family":"Felinto de Brito","sequence":"first","affiliation":[{"name":"Centro de Pesquisas Aggeu Magalh\u00e3es, FIOCRUZ-PE, Campus da Universidade Federal de Pernambuco s\/n, Cidade Universit\u00e1ria, 50.670-901 Recife, PE, Brazil"},{"name":"Centro de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Pernambuco s\/n, Cidade Universit\u00e1ria, 50.670-901 Recife, PE, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria 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