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An extracellular lipase of <jats:italic>Pseudozyma hubeiensis<\/jats:italic> (strain HB85A) was immobilized by adsorption onto a polystyrene-divinylbenzene support. Furthermore, response surface methodology (RSM) was employed to optimize enzyme immobilization and evaluate the optimum temperature and pH for free and immobilized enzyme. The optimal immobilization conditions observed were 150\u2009min incubation time, pH 4.76, and an enzyme\/support ratio of 1282\u2009U\/g support. Optimal activity temperature for free and immobilized enzyme was found to be 68\u00b0C and 52\u00b0C, respectively. Optimal activity pH for free and immobilized lipase was pH 4.6 and 6.0, respectively. 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