{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T07:01:19Z","timestamp":1771657279943,"version":"3.50.1"},"reference-count":23,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Astellas"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Analytical Methods in Chemistry"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>Moxifloxacin is a broad-spectrum antibacterial 8-methoxy-fluoroquinolone. In order to evaluate the pharmacokinetic properties of moxifloxacin in mouse plasma and brain tissue, we developed a high-performance liquid chromatography (HPLC) method. This study was based on single-drug delivery, intravenously dosed in a central listeriosis murine model. The method employed a reversed-phase Lichrospher RP-18 with a precolumn (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mn>250<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:mn>4.6<\/mml:mn><\/mml:math>\u2009mm) and a mobile phase composed of a mixture of acetonitrile, methanol, and citric buffer (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mtext>pH<\/mml:mtext><mml:mo>=<\/mml:mo><mml:mn>3.5<\/mml:mn><\/mml:math>) with sodium dodecyl sulfate and tetrabutylammonium bromide. Fluorescence detection was performed at an excitation wavelength of 290\u2009nm and an emission wavelength of 550\u2009nm. The relative standard deviation of intra- and inter-day assays was &lt;10%. This validated method led to a short retention time (8.0\u2009min) for moxifloxacin. The standard curves were linear from 5\u2013250\u2009<jats:italic>\u03bc<\/jats:italic>g\/L in plasma and from 0.1\u20132.5\u2009<jats:italic>\u03bc<\/jats:italic>g\/g of brain tissue. The limits of quantification were 5\u2009<jats:italic>\u03bc<\/jats:italic>g\/L in plasma and 0.1\u2009<jats:italic>\u03bc<\/jats:italic>g\/g in brain tissue. The method enabled the detection of systemic antimicrobial in plasma and in CNS in<jats:italic>Listeria<\/jats:italic>-infected mice. Injected moxifloxacin passed through the encephalic barrier within a 30 to 60 min after injection time frame. Moxifloxacin pharmacokinetics are modeled in an infected model compared to control mice.<\/jats:p>","DOI":"10.1155\/2012\/436349","type":"journal-article","created":{"date-parts":[[2012,3,14]],"date-time":"2012-03-14T21:02:14Z","timestamp":1331758934000},"page":"1-7","source":"Crossref","is-referenced-by-count":9,"title":["High-Performance Liquid Chromatography Assay for Moxifloxacin in Brain Tissue and Plasma: Validation in a Pharmacokinetic Study in a Murine Model of Cerebral Listeriosis"],"prefix":"10.1155","volume":"2012","author":[{"given":"Renaud","family":"Respaud","sequence":"first","affiliation":[{"name":"Laboratoire de Suivi Th\u00e9rapeutique et de Contr\u00f4le des M\u00e9dicaments, Service de Pharmacie, Assistance Publique-H\u00f4pitaux de Paris (AP-HP), H\u00f4pital Necker-Enfants Malades, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Solene","family":"Grayo","sequence":"additional","affiliation":[{"name":"Laboratoire de Listeria, 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