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However, effective therapeutic drugs against EV71 and CVA16 are rare.<jats:italic>Kalanchoe gracilis<\/jats:italic>(L.) DC is used for the treatment of injuries, pain, and inflammation. This study investigated antiviral effects of<jats:italic>K. gracilis<\/jats:italic>leaf extract on EV71 and CVA16 replications. HPLC analysis with a C-18 reverse phase column showed fingerprint profiles of<jats:italic>K. gracilis<\/jats:italic>leaf extract had 15 chromatographic peaks. UV\/vis absorption spectra revealed peaks 5, 12, and 15 as ferulic acid, quercetin, and kaempferol, respectively.<jats:italic>K. gracilis<\/jats:italic>leaf extract showed little cytotoxicity, but exhibited concentration-dependent antiviral activities including cytopathic effect, plaque, and virus yield reductions.<jats:italic>K. gracilis<\/jats:italic>leaf extract was shown to be more potent in antiviral activity than ferulic acid, quercetin, and kaempferol, significantly inhibiting<jats:italic>in vitro<\/jats:italic>replication of EV71 (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:msub><mml:mtext>IC<\/mml:mtext><mml:mrow><mml:mtext>50<\/mml:mtext><\/mml:mrow><\/mml:msub><mml:mo>=<\/mml:mo><mml:mn>35.88<\/mml:mn><\/mml:math>\u2009\u03bcg\/mL) and CVA16 (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:msub><mml:mtext>IC<\/mml:mtext><mml:mrow><mml:mtext>50<\/mml:mtext><\/mml:mrow><\/mml:msub><mml:mo>=<\/mml:mo><mml:mn>42.91<\/mml:mn><\/mml:math>\u2009\u03bcg\/mL). Moreover,<jats:italic>K. gracilis<\/jats:italic>leaf extract is a safe antienteroviral agent with the inactivation of viral 2A protease and reduction of IL-6 and RANTES expressions.<\/jats:p>","DOI":"10.1155\/2012\/503165","type":"journal-article","created":{"date-parts":[[2012,5,15]],"date-time":"2012-05-15T17:10:34Z","timestamp":1337101834000},"page":"1-13","source":"Crossref","is-referenced-by-count":13,"title":["Antiviral Ability of<i>Kalanchoe gracilis<\/i>Leaf Extract against Enterovirus 71 and Coxsackievirus A16"],"prefix":"10.1155","volume":"2012","author":[{"given":"Ching-Ying","family":"Wang","sequence":"first","affiliation":[{"name":"School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan"},{"name":"Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, 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