{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T10:39:04Z","timestamp":1780569544731,"version":"3.54.1"},"reference-count":55,"publisher":"Wiley","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI 088650"],"award-info":[{"award-number":["AI 088650"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["AI 088650"],"award-info":[{"award-number":["AI 088650"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Parasitology Research"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>High levels of proinflammatory cytokines such as IFN-<jats:italic>\u03b3<\/jats:italic>and TNF are associated with tissue lesions in cutaneous leishmaniasis (CL). We previously demonstrated that<jats:italic>Schistosoma mansoni<\/jats:italic>antigens downmodulate the<jats:italic>in vitro<\/jats:italic>cytokine response in CL. In the current study we evaluated whether<jats:italic>S. mansoni<\/jats:italic>antigens alter monocyte and T-lymphocyte phenotypes in leishmaniasis. Peripheral blood mononuclear cells of CL patients were cultured with<jats:italic>L. braziliensis<\/jats:italic>antigen in the presence or absence of the<jats:italic>S. mansoni<\/jats:italic>antigens rSm29, rSmTSP-2- and PIII. Cells were stained with fluorochrome conjugated antibodies and analyzed by flow cytometry. The addition of rSm29 to the cultures decreased the expression of HLA-DR in nonclassical (CD14<jats:sup>+<\/jats:sup>CD16<jats:sup>++<\/jats:sup>) monocytes, while the addition of PIII diminished the expression of this molecule in classical (CD14<jats:sup>++<\/jats:sup>CD16<jats:sup>-<\/jats:sup>) and intermediate (CD14<jats:sup>++<\/jats:sup>CD16<jats:sup>+<\/jats:sup>) monocytes. The addition of PIII and rSmTSP-2 resulted in downmodulation of CD80 expression in nonclassical and CD86 expression in intermediate monocytes, respectively. These two antigens increased the expression of CTLA-4 in CD4<jats:sup>+<\/jats:sup>T cells and they also expanded the frequency of CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>high<\/jats:sup>Foxp3<jats:sup>+<\/jats:sup>T cells. Taken together, we show that<jats:italic>S. mansoni<\/jats:italic>antigens, mainly rSmTSP-2 and PIII, are able to decrease the activation status of monocytes and also to upregulate the expression of modulatory molecules in T lymphocytes.<\/jats:p>","DOI":"10.1155\/2012\/520308","type":"journal-article","created":{"date-parts":[[2012,11,13]],"date-time":"2012-11-13T21:00:28Z","timestamp":1352840428000},"page":"1-10","source":"Crossref","is-referenced-by-count":9,"title":["Changes in T-Cell and Monocyte Phenotypes<i>In Vitro<\/i>by<i>Schistosoma mansoni<\/i>Antigens in Cutaneous Leishmaniasis Patients"],"prefix":"10.1155","volume":"2012","author":[{"given":"Aline Michelle Barbosa","family":"Bafica","sequence":"first","affiliation":[{"name":"Servi\u00e7o de Imunologia, Complexo Hospitalar Universit\u00e1rio Professor Edgard Santos, Universidade Federal da Bahia, 5 Rua Jo\u00e3o das Botas s\/n, Canela, 40110-160 Salvador, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana Santos","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Imunologia, Complexo Hospitalar Universit\u00e1rio Professor Edgard Santos, Universidade Federal da Bahia, 5 Rua Jo\u00e3o das Botas s\/n, Canela, 40110-160 Salvador, BA, Brazil"},{"name":"Departamento de Ci\u00eancias da Vida, Universidade do Estado da Bahia, 2555 Rua Silveira Martins, Cabula, 41.150-000 Salvador, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio Costa","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, 6627 Avenida Ant\u00f4nio Carlos, Pampulha, 31270-901 Belo Horizonte, MG, Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Doen\u00e7as Tropicais (INCT-DT-CNPQ\/MCT), Rua Jo\u00e3o das Botas s\/n, Canela, 40110-160 Salvador, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Loukas","sequence":"additional","affiliation":[{"name":"Queensland Tropical Health Alliance and School of Public Health and Tropical Medicine, James Cook University, Cairns, QLD 4878, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo","family":"G\u00f3es","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica e Imunologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, 6627 Avenida Ant\u00f4nio Carlos, Pampulha, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Riccio","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Imunologia, Complexo Hospitalar Universit\u00e1rio Professor Edgard Santos, Universidade Federal da Bahia, 5 Rua Jo\u00e3o das Botas s\/n, Canela, 40110-160 Salvador, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar M.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Imunologia, Complexo Hospitalar Universit\u00e1rio Professor Edgard Santos, Universidade Federal da Bahia, 5 Rua Jo\u00e3o das Botas s\/n, Canela, 40110-160 Salvador, BA, Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Doen\u00e7as Tropicais (INCT-DT-CNPQ\/MCT), Rua Jo\u00e3o das Botas s\/n, Canela, 40110-160 Salvador, BA, Brazil"},{"name":"Escola Bahiana de Medicina e Sa\u00fade P\u00fablica, No. 275 Avenida Dom Jo\u00e3o VI, Brotas, 40290-000 Salvador, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Ilma","family":"Araujo","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Imunologia, Complexo Hospitalar Universit\u00e1rio Professor Edgard Santos, Universidade Federal da Bahia, 5 Rua Jo\u00e3o das Botas s\/n, Canela, 40110-160 Salvador, BA, Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Doen\u00e7as Tropicais (INCT-DT-CNPQ\/MCT), Rua Jo\u00e3o das Botas s\/n, Canela, 40110-160 Salvador, BA, Brazil"},{"name":"Escola Bahiana de Medicina e Sa\u00fade P\u00fablica, No. 275 Avenida Dom Jo\u00e3o VI, Brotas, 40290-000 Salvador, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/0738-081X(96)00057-0"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.1.508-514.2004"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.12.6734-6740.2002"},{"issue":"1","key":"4","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1590\/S0100-879X1998000100020","volume":"31","year":"1998","journal-title":"Brazilian Journal of Medical and Biological Research"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxg012"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.1999.00213.x"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2006.00431.x"},{"key":"8","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1590\/S0074-02762011000700012","volume":"106","year":"2011","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz"},{"key":"9","first-page":"73","volume":"100, part 1","year":"1990","journal-title":"Parasitology"},{"issue":"4","key":"10","first-page":"609","volume":"5","year":"2006","journal-title":"Genetics and Molecular Research"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1038\/nm1430"},{"issue":"6","key":"12","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1017\/S0031182000066105","volume":"112","year":"1996","journal-title":"Parasitology"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182097001078"},{"key":"14","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1002\/ddr.20459","volume":"72","year":"2011","journal-title":"Drug Development Research"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-02-258558"},{"key":"16","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1182\/blood-2010-12-326355","volume":"118","year":"2011","journal-title":"Blood"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.08.012"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017484"},{"key":"19","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1111\/j.1365-2036.2011.04807.x","volume":"34","year":"2011","journal-title":"Alimentary Pharmacology & Therapeutics"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1097\/QAI.0b013e3181c1d4fe"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2009.03224.x"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0105040"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.2.631"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2008.11.017"},{"issue":"5","key":"25","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/1074-7613(94)90071-X","volume":"1","year":"1994","journal-title":"Immunity"},{"issue":"6127","key":"26","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/328267a0","volume":"328","year":"1987","journal-title":"Nature"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2004.09.015"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.2.665-672.2001"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxh165"},{"issue":"3","key":"30","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.4049\/jimmunol.167.3.1245","volume":"167","year":"2001","journal-title":"Journal of Immunology"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200526069"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.021908.132723"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1038\/nature01152"},{"issue":"3","key":"34","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1172\/JCI114493","volume":"85","year":"1990","journal-title":"Journal of Clinical Investigation"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-6-1"},{"key":"36","volume":"6, article e1564","year":"2011","journal-title":"PLOS Neglected Tropical Diseases"},{"issue":"11","key":"37","doi-asserted-by":"crossref","first-page":"E54","DOI":"10.1086\/340261","volume":"34","year":"2002","journal-title":"Clinical Infectious Diseases"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00890-06"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2005.01707.x"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1086\/315071"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1086\/425017"},{"issue":"5","key":"42","doi-asserted-by":"crossref","first-page":"3157","DOI":"10.4049\/jimmunol.172.5.3157","volume":"172","year":"2004","journal-title":"Journal of Immunology"},{"issue":"5","key":"43","first-page":"27","volume":"99","year":"2004","journal-title":"Memorias do Instituto Oswaldo Cruz"},{"key":"44","first-page":"671","volume":"64","year":"2011","journal-title":"Arthritis & Rheumatism"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0209082"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0806510"},{"key":"47","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s12026-012-8297-3","volume":"53","year":"2012","journal-title":"Immunology Research"},{"issue":"7","key":"48","doi-asserted-by":"crossref","first-page":"2527","DOI":"10.1182\/blood.V74.7.2527.2527","volume":"74","year":"1989","journal-title":"Blood"},{"key":"49","doi-asserted-by":"crossref","first-page":"24","DOI":"10.2174\/187153012799278929","volume":"12","year":"2012","journal-title":"Endocrine, Metabolic & Immune Disorders"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00783-07"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1086\/509808"},{"issue":"2","key":"52","doi-asserted-by":"crossref","first-page":"87","DOI":"10.4269\/ajtmh.2001.65.87","volume":"65","year":"2001","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1086\/424848"},{"issue":"1","key":"54","doi-asserted-by":"crossref","first-page":"79","DOI":"10.4269\/ajtmh.2005.73.79","volume":"73","year":"2005","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1086\/315082"}],"container-title":["Journal of Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jpr\/2012\/520308.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jpr\/2012\/520308.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jpr\/2012\/520308.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T02:41:55Z","timestamp":1594867315000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/jpr\/2012\/520308\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":55,"alternative-id":["520308","520308"],"URL":"https:\/\/doi.org\/10.1155\/2012\/520308","relation":{},"ISSN":["2090-0023","2090-0031"],"issn-type":[{"value":"2090-0023","type":"print"},{"value":"2090-0031","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}