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IFN-<jats:italic>\u03b3<\/jats:italic>, IL-12, and CD8+ T lymphocytes are important components of host immune responses against<jats:italic>B. abortus<\/jats:italic>. Herein, IFN-<jats:italic>\u03b3<\/jats:italic>and IL-12\/<jats:italic>\u03b2<\/jats:italic>2-microglobulin (<jats:italic>\u03b2<\/jats:italic>2-m) knockout mice were used to determine whether CD8+ T cells and IL-12-dependent IFN-<jats:italic>\u03b3<\/jats:italic>deficiency would be more critical to control<jats:italic>B. abortus<\/jats:italic>infection compared to the lack of endogenous IFN-<jats:italic>\u03b3<\/jats:italic>. At 1 week after infection, IFN-<jats:italic>\u03b3<\/jats:italic>KO and IL-12\/<jats:italic>\u03b2<\/jats:italic>2-m KO mice showed increased numbers of bacterial load in spleens; however, at 3 weeks postinfection (p.i.), only IFN-<jats:italic>\u03b3<\/jats:italic>KO succumbed to<jats:italic>Brucella<\/jats:italic>. All IFN-<jats:italic>\u03b3<\/jats:italic>KO had died at 16 days p.i. whereas death within the IL-12\/<jats:italic>\u03b2<\/jats:italic>2-m KO group was delayed and occurred at 32 days until 47 days postinfection. Susceptibility of IL-12\/<jats:italic>\u03b2<\/jats:italic>2-m KO animals to<jats:italic>Brucella<\/jats:italic>was associated to undetectable levels of IFN-<jats:italic>\u03b3<\/jats:italic>in mouse splenocytes and inability of these cells to lyse<jats:italic>Brucella<\/jats:italic>-infected macrophages. However, the lack of endogenous IFN-<jats:italic>\u03b3<\/jats:italic>was found to be more important to control brucellosis than CD8+ T cells and IL-12-dependent IFN-<jats:italic>\u03b3<\/jats:italic>deficiencies.<\/jats:p>","DOI":"10.1155\/2012\/589494","type":"journal-article","created":{"date-parts":[[2011,12,11]],"date-time":"2011-12-11T21:00:45Z","timestamp":1323637245000},"page":"1-7","source":"Crossref","is-referenced-by-count":46,"title":["Host Susceptibility to<i>Brucella abortus<\/i>Infection Is More Pronounced in IFN-<b><i>\u03b3<\/i><\/b>knockout than IL-12\/<b><i>\u03b2<\/i><\/b>2-Microglobulin Double-Deficient Mice"],"prefix":"10.1155","volume":"2012","author":[{"given":"Ana Paula M. S.","family":"Brand\u00e3o","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda S.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia B.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leda Q.","family":"Vieira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, 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