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Our aim was to determine whether mitochondrial dysfunction leading to hepatic necrosis and apoptosis after hemorrhage\/resuscitation (H\/R) was dependent on JNK2. Under pentobarbital anesthesia, wildtype (WT) and JNK2 deficient (KO) mice were hemorrhaged to 30\u2009mm\u2009Hg for 3\u2009h and then resuscitated with shed blood plus half the volume of lactated Ringer\u2019s solution. Serum alanine aminotransferase (ALT), necrosis, apoptosis and oxidative stress were assessed 6\u2009h after resuscitation. Mitochondrial polarization was assessed by intravital microscopy. After H\/R, ALT in WT-mice increased from 130\u2009U\/L to 4800\u2009U\/L. In KO-mice, ALT after H\/R was blunted to 1800\u2009U\/l (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:mrow><\/mml:math>). Necrosis, caspase-3 activity and ROS were all substantially decreased in KO compared to WT mice after H\/R. After sham operation, intravital microscopy revealed punctate mitochondrial staining by rhodamine 123 (Rh123), indicating normal mitochondrial polarization. At 4\u2009h after H\/R, Rh123 staining became dim and diffuse in 58% of hepatocytes, indicating depolarization and onset of the mitochondrial permeability transition (MPT). By contrast, KO mice displayed less depolarization after H\/R (23%, <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:mrow><\/mml:math>). In conclusion, JNK2 contributes to MPT-mediated liver injury after H\/R.<\/jats:p>","DOI":"10.1155\/2012\/641982","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T22:10:16Z","timestamp":1340835016000},"page":"1-9","source":"Crossref","is-referenced-by-count":4,"title":["C-Jun N-Terminal Kinase 2 Promotes Liver Injury via the Mitochondrial Permeability Transition after Hemorrhage and Resuscitation"],"prefix":"10.1155","volume":"2012","author":[{"given":"Christoph","family":"Czerny","sequence":"first","affiliation":[{"name":"Center for Cell Death, Injury & Regeneration, Departments of Pharmaceutical & Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425, USA"},{"name":"Departement of Trauma Surgery, J.W. 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