{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T06:41:23Z","timestamp":1771051283867,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2011-0018631"],"award-info":[{"award-number":["2011-0018631"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["10039303"],"award-info":[{"award-number":["10039303"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002994","name":"Ministry of Knowledge Economy","doi-asserted-by":"publisher","award":["2011-0018631"],"award-info":[{"award-number":["2011-0018631"]}],"id":[{"id":"10.13039\/501100002994","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002994","name":"Ministry of Knowledge Economy","doi-asserted-by":"publisher","award":["10039303"],"award-info":[{"award-number":["10039303"]}],"id":[{"id":"10.13039\/501100002994","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>DA-9701 is a new botanical drug composed of the extracts of Corydalis tuber and Pharbitidis semen, and it is used as an oral therapy for the treatment of functional dyspepsia in Korea. The inhibitory potentials of DA-9701 and its component herbs, Corydalis tuber and Pharbitidis semen, on the activities of seven major human cytochrome P450 (CYP) enzymes and four UDP-glucuronosyltransferase (UGT) enzymes in human liver microsomes were investigated using liquid chromatography-tandem mass spectrometry. DA-9701 and Corydalis tuber extract slightly inhibited UGT1A1-mediated etoposide glucuronidation, with 50% inhibitory concentration (IC<jats:sub>50<\/jats:sub>) values of 188 and 290\u2009\u03bcg\/mL, respectively. DA-9701 inhibited CYP2D6-catalyzed bufuralol<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:msup><mml:mn>1<\/mml:mn><mml:mo>\u2032<\/mml:mo><\/mml:msup><\/mml:math>-hydroxylation with an inhibition constant (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:msub><mml:mi>K<\/mml:mi><mml:mtext>i<\/mml:mtext><\/mml:msub><\/mml:math>) value of 6.3\u2009\u03bcg\/mL in a noncompetitive manner. Corydalis tuber extract competitively inhibited CYP2D6-mediated bufuralol<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:msup><mml:mn>1<\/mml:mn><mml:mo>\u2032<\/mml:mo><\/mml:msup><\/mml:math>-hydroxylation, with a<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:msub><mml:mi>K<\/mml:mi><mml:mtext>i<\/mml:mtext><\/mml:msub><\/mml:math>value of 3.7\u2009\u03bcg\/mL, whereas Pharbitidis semen extract showed no inhibition. The volume in which the dose could be diluted to generate an IC<jats:sub>50<\/jats:sub>equivalent concentration (volume per dose index) value of DA-9701 for inhibition of CYP2D6 activity was 1.16\u2009L\/dose, indicating that DA-9701 may not be a potent CYP2D6 inhibitor. Further clinical studies are warranted to evaluate the<jats:italic>in vivo<\/jats:italic>extent of the observed<jats:italic>in vitro<\/jats:italic>interactions.<\/jats:p>","DOI":"10.1155\/2012\/650718","type":"journal-article","created":{"date-parts":[[2012,4,2]],"date-time":"2012-04-02T21:01:24Z","timestamp":1333400484000},"page":"1-7","source":"Crossref","is-referenced-by-count":7,"title":["Effect of a New Prokinetic Agent DA-9701 Formulated with Corydalis Tuber and Pharbitidis Semen on Cytochrome P450 and UDP-Glucuronosyltransferase Enzyme Activities in Human Liver Microsomes"],"prefix":"10.1155","volume":"2012","author":[{"given":"Hye Young","family":"Ji","sequence":"first","affiliation":[{"name":"Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy, The Catholic University of Korea, 43-1 Yeokgok 2-dong, Wonmi-gu, Gyeonggi-do, Bucheon, 420-743, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwang Hyeon","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji Hyeon","family":"Jeong","sequence":"additional","affiliation":[{"name":"Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy, The Catholic University of Korea, 43-1 Yeokgok 2-dong, Wonmi-gu, Gyeonggi-do, Bucheon, 420-743, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dae-Young","family":"Lee","sequence":"additional","affiliation":[{"name":"Research Center, Dong-A Pharmaceutical Co., Yongin 446-905, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun Joo","family":"Shim","sequence":"additional","affiliation":[{"name":"Research Center, Dong-A Pharmaceutical Co., Yongin 446-905, Republic of 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