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As one of the key characteristics of modulation instability, the most unstable condition attracts lots of attention. The most unstable condition is investigated here with two kinds of initial wave systems via a numerical high\u2010order spectral method (HOS) for surface water wave field. Classically, one carrier wave and a pair of sidebands are implied as the first kind of initial wave system: \u201cseeded\u201d wave system. In the second kind of initial wave system: \u201cun\u2010seeded\u201d wave system, only one carrier wave is implied. Two impressive new results are present. One result shows that the grow rates of lower and upper sideband are different within the \u201cseeded\u201d wave system. It means that, for a given wave steepness, the most unstable lower sideband is not in pair with the most unstable upper sideband. Another result shows the fastest growing sidebands are exactly in pair from \u201cunseeded\u201d wave system. 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