{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:46:45Z","timestamp":1787017605529,"version":"build-2736575974"},"reference-count":82,"publisher":"Wiley","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["0315097B"],"award-info":[{"award-number":["0315097B"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["ECS-0210332"],"award-info":[{"award-number":["ECS-0210332"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["0315097B"],"award-info":[{"award-number":["0315097B"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["ECS-0210332"],"award-info":[{"award-number":["ECS-0210332"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biomedicine and Biotechnology"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>\n                    Microfabricated thermoelectric controllers can be employed to investigate mechanisms underlying myosin-driven sliding of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -regulated actin and disease-associated mutations in myofilament proteins. Specifically, we examined actin filament sliding\u2014with or without human cardiac troponin (Tn) and \u03b1-tropomyosin (Tm)\u2014propelled by rabbit skeletal heavy meromyosin, when temperature was varied continuously over a wide range (\u223c20\u201363\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\">\n                      <mml:mrow>\n                        <mml:mtext>C<\/mml:mtext>\n                        <mml:mo>\u00b0<\/mml:mo>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    ). At the upper end of this temperature range, reversible dysregulation of thin filaments occurred at pCa 9 and 5; actomyosin function was unaffected. Tn-Tm enhanced sliding speed at pCa 5 and increased a transition temperature (\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\">\n                      <mml:mrow>\n                        <mml:msub>\n                          <mml:mi>T<\/mml:mi>\n                          <mml:mi>t<\/mml:mi>\n                        <\/mml:msub>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    ) between a high activation energy (\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\">\n                      <mml:mrow>\n                        <mml:msub>\n                          <mml:mi>E<\/mml:mi>\n                          <mml:mi>a<\/mml:mi>\n                        <\/mml:msub>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    ) but low temperature regime and a low\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\">\n                      <mml:mrow>\n                        <mml:msub>\n                          <mml:mi>E<\/mml:mi>\n                          <mml:mi>a<\/mml:mi>\n                        <\/mml:msub>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    but high temperature regime. This was modulated by factors that alter cross-bridge number and kinetics. Three familial hypertrophic cardiomyopathy (FHC) mutations, cTnI R145G, cTnI K206Q, and cTnT R278C, cause dysregulation at temperatures \u223c5\u20138\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\">\n                      <mml:mrow>\n                        <mml:mtext>C<\/mml:mtext>\n                        <mml:mo>\u00b0<\/mml:mo>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    lower; the latter two increased speed at pCa 5 at all temperatures.\n                  <\/jats:p>","DOI":"10.1155\/2012\/657523","type":"journal-article","created":{"date-parts":[[2012,2,14]],"date-time":"2012-02-14T16:02:27Z","timestamp":1329235347000},"page":"1-13","source":"Crossref","is-referenced-by-count":16,"title":["Micromechanical Thermal Assays of Ca\n                    <sup>2+<\/sup>\n                    -Regulated Thin-Filament Function and Modulation by Hypertrophic Cardiomyopathy Mutants of Human Cardiac Troponin"],"prefix":"10.1155","volume":"2012","author":[{"given":"Nicolas M.","family":"Brunet","sequence":"first","affiliation":[{"name":"Department of Biological Science, The Florida State University, P.O. Box 3064295, Tallahassee, FL 32306, USA"},{"name":"Institute of Molecular Biophysics, The Florida State University, Tallahassee, FL 32306, USA"},{"name":"Donders Institute for Brain, Cognition and Behavior, Centre for Cognitive Neuroimaging, Radboud University Nijmegen, 6500 HB Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Goran","family":"Mihajlovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Physics, The Florida State University, Tallahassee, FL 32306, USA"},{"name":"San Jose Research Center, Hitachi Global Storage Technologies, San Jose, CA 95135, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khaled","family":"Aledealat","sequence":"additional","affiliation":[{"name":"Department of Physics, The Florida State University, Tallahassee, FL 32306, USA"},{"name":"Department of Physics, Jordan University of Science and Technology, Irbid 22110, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biological Science, The Florida State University, P.O. Box 3064295, Tallahassee, FL 32306, USA"},{"name":"Department of Neurobiology, College of Basic Medical Science, Southern Medical University, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Physics, The Florida State University, Tallahassee, FL 32306, USA"},{"name":"Integrative NanoScience Institute (INSI), The Florida State University, Tallahassee, FL 32306, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"von Moln\u00e1r","sequence":"additional","affiliation":[{"name":"Department of Physics, The Florida State University, Tallahassee, FL 32306, USA"},{"name":"Integrative NanoScience Institute (INSI), The Florida State University, Tallahassee, FL 32306, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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