{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T10:43:04Z","timestamp":1740134584566,"version":"3.37.3"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100005089","name":"Beijing Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["7112012","KM200910025003"],"award-info":[{"award-number":["7112012","KM200910025003"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002888","name":"Beijing Municipal Commission of Education","doi-asserted-by":"publisher","award":["7112012","KM200910025003"],"award-info":[{"award-number":["7112012","KM200910025003"]}],"id":[{"id":"10.13039\/501100002888","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biomedicine and Biotechnology"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>Hyperhomocysteinemia (HHcy) is a well-known independent risk factor for vascular diseases in the general population. This study was to explore the effect of genistein (GST), a natural bioactive compound derived from legumes, on HHcy-induced vascular endothelial impairment in ovariectomized rats<jats:italic>in vivo<\/jats:italic>. Thirty-two adult female Wistar rats were assigned randomly into four groups (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>8<\/mml:mn><\/mml:mrow><\/mml:math>): (a) Con: control; (b) Met: 2.5% methionine diet; (c) OVX + Met: ovariectomy + 2.5% methionine diet; (d) OVX + Met + GST: ovariectomy + 2.5% methionine diet + supplementation with genistein. After 12\u2009wk of different treatment, the rats' blood, toracic aortas and liver samples were collected for analysis. Results showed that high-methionine diet induced both elevation of plasma Hcy and endothelial dysfunction, and ovariectomy deteriorated these injuries. Significant improvement of both functional and morphological changes of vascular endothelium was observed in OVX + Met + GST group; meanwhile the plasma Hcy levels decreased remarkably. There were significant elevations of plasma ET-1 and liver MDA levels in ovariectomized HHcy rats, and supplementation with genistein could attenuate these changes. These results implied that genistein could lower the elevated Hcy levels, and prevent the development of endothelial impairment in ovariectomized HHcy rats. This finding may shed a novel light on the anti-atherogenic activities of genistein in HHcy patients.<\/jats:p>","DOI":"10.1155\/2012\/730462","type":"journal-article","created":{"date-parts":[[2012,11,11]],"date-time":"2012-11-11T01:32:06Z","timestamp":1352597526000},"page":"1-10","source":"Crossref","is-referenced-by-count":9,"title":["Genistein Attenuates Vascular Endothelial Impairment in Ovariectomized Hyperhomocysteinemic Rats"],"prefix":"10.1155","volume":"2012","author":[{"given":"Panpan","family":"Zhen","sequence":"first","affiliation":[{"name":"Department of Pathology, The Luhe Teaching Hospital of the Capital Medical University, 82 Xinhuanan Road, Tongzhou District, Beijing 101149, China"},{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, You An Men Wai, Fengtai District, Beijing 100069, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, You An Men Wai, Fengtai District, Beijing 100069, China"},{"name":"The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing An Zhen Hospital, Capital Medical University, Ministry of Education, 2 Anzhen Road, Chaoyang District, Beijing 100029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dandan","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, You An Men Wai, Fengtai District, Beijing 100069, China"},{"name":"The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing An Zhen Hospital, Capital Medical University, Ministry of Education, 2 Anzhen Road, Chaoyang 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