{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T21:41:47Z","timestamp":1769550107234,"version":"3.49.0"},"reference-count":24,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Medical Science and Technology Research Project of Henan Province, China","award":["200903109"],"award-info":[{"award-number":["200903109"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biomedicine and Biotechnology"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p><jats:italic>Aim<\/jats:italic>. To investigate the anti-tumor effects and mechanisms of gecko crude peptides (GCPs)<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>.<jats:italic>Methods<\/jats:italic>. 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay was applied to measure the effects of GCPs on the HepG2 cell viability. Fluorescence morphology was used to identify apoptotic cells. A xenograft H22 liver cancer model was established in Kunming mice. The tumor-bearing mice were treated with daily intraperitoneal injections of normal saline (NS group) or GCPs (80, 40 or 20\u2009mg\/kg) for 10 days, or once per two days with 2\u2009mg\/kg doxorubicin (ADR group;<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>10<\/mml:mn><\/mml:mrow><\/mml:math>each). Serum tumor necrosis factor (TNF-<jats:italic>\u03b1<\/jats:italic>) and interleukin (IL)-6 were quantified using ELISA assay.<jats:italic>Results<\/jats:italic>. GCPs significantly inhibited the growth of HepG2 cells and induced typical apoptotic morphological features through increasing bcl-2\/bax ratio in a dose- and time-dependent manner<jats:italic>in vitro<\/jats:italic>. The tumor weights of the ADR group, GCPs (H) group, GCPs (M) group, GCPs (L) group were smaller compared to the NS group. While the white blood cell count, thymus index, spleen index were higher in the high dose GCPs group than the NS group (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:mrow><\/mml:math>), the VEGF expression in tumor tissue and serum TNF-<jats:italic>\u03b1<\/jats:italic>and IL-6 levels in the GCPs groups were lower than the NS group (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:mrow><\/mml:math>).<\/jats:p>","DOI":"10.1155\/2012\/743573","type":"journal-article","created":{"date-parts":[[2012,10,3]],"date-time":"2012-10-03T21:06:08Z","timestamp":1349298368000},"page":"1-6","source":"Crossref","is-referenced-by-count":13,"title":["Gecko Crude Peptides Induce Apoptosis in Human Liver Carcinoma Cells<i>In Vitro<\/i>and Exert Antitumor Activity in a Mouse Ascites H22 Xenograft Model"],"prefix":"10.1155","volume":"2012","author":[{"given":"Ying","family":"Song","sequence":"first","affiliation":[{"name":"Medical College, Henan University of Science & Technology, Luoyang 471003, Henan Province, China"},{"name":"The 1st Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, Henan Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Gang","family":"Wang","sequence":"additional","affiliation":[{"name":"Medical College, Henan University of Science & Technology, Luoyang 471003, Henan Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui-Fang","family":"Li","sequence":"additional","affiliation":[{"name":"Medical College, Henan University of Science & Technology, Luoyang 471003, Henan Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Medical College, Henan University of Science & Technology, Luoyang 471003, Henan Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao-Chu","family":"Cui","sequence":"additional","affiliation":[{"name":"Medical College, Henan University of Science & Technology, Luoyang 471003, Henan Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leng-Xin","family":"Duan","sequence":"additional","affiliation":[{"name":"Medical College, Henan University of Science & Technology, Luoyang 471003, Henan Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Lu","sequence":"additional","affiliation":[{"name":"The 1st Affiliated Hospital of Henan University of Science and Technology, Luoyang 471003, Henan Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"98","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6604477"},{"issue":"1","key":"2","first-page":"13","volume":"20","year":"2008","journal-title":"Oncology Reports"},{"issue":"4","key":"3","first-page":"217","volume":"30","year":"2009","journal-title":"Chinese Journal of Biochemical Pharmaceutics"},{"issue":"3","key":"4","first-page":"286","volume":"45","year":"2007","journal-title":"Journal of Shandong Univercity"},{"issue":"2","key":"5","first-page":"178","volume":"27","year":"2007","journal-title":"Zhongguo Zhongxiyi Jiehe Zazhi"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1007\/BF02857438"},{"issue":"8","key":"7","first-page":"759","volume":"26","year":"2006","journal-title":"Zhongguo Zhongxiyi Jiehe Zazhi"},{"issue":"4","key":"8","first-page":"281","volume":"32","year":"2007","journal-title":"Zhongguo Zhongyao Zazhi"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.14.3990"},{"issue":"8","key":"10","first-page":"1213","volume":"33","year":"2010","journal-title":"Journal of Chinese Medicinal Materials"},{"key":"11","first-page":"2428","volume":"14","year":"2006","journal-title":"Chinese Journal of Digestion"},{"key":"12","first-page":"502","volume":"19","year":"1999","journal-title":"Zhongguo Zhongxiyi Jiehe Za Zhi"},{"issue":"1","key":"13","first-page":"58","volume":"26","year":"2006","journal-title":"Zhongguo Zhongxiyi Jiehe Zazhi"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2007.04.031"},{"issue":"29","key":"15","doi-asserted-by":"crossref","first-page":"4011","DOI":"10.3748\/wjg.v13.i29.4011","volume":"13","year":"2007","journal-title":"World Journal of Gastroenterology"},{"issue":"14","key":"21","doi-asserted-by":"crossref","first-page":"2024","DOI":"10.3748\/wjg.v10.i14.2024","volume":"10","year":"2004","journal-title":"World Journal of Gastroenterology"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.06.004"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.48.34096"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1021\/jf0730744"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2006.07.011"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2003.09.034"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(99)00198-8"},{"issue":"7","key":"23","first-page":"964","volume":"24","year":"2008","journal-title":"Chinese Pharmacological Bulletin"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1038\/nrc883"}],"container-title":["Journal of Biomedicine and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2012\/743573.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2012\/743573.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2012\/743573.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,21]],"date-time":"2017-06-21T00:58:35Z","timestamp":1498006715000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2012\/743573\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":24,"alternative-id":["743573","743573"],"URL":"https:\/\/doi.org\/10.1155\/2012\/743573","relation":{},"ISSN":["1110-7243","1110-7251"],"issn-type":[{"value":"1110-7243","type":"print"},{"value":"1110-7251","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}