{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T16:53:56Z","timestamp":1775840036502,"version":"3.50.1"},"reference-count":81,"publisher":"Wiley","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o Butantan, Brazil"},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biomedicine and Biotechnology"],"published-print":{"date-parts":[[2012]]},"abstract":"<jats:p>Leptospirosis is considered a neglected infectious disease of human and veterinary concern. Although extensive investigations on host-pathogen interactions have been pursued by several research groups, mechanisms of infection, invasion and persistence of pathogenic<jats:italic>Leptospira<\/jats:italic>spp. remain to be elucidated. We have reported the ability of leptospires to bind human plasminogen (PLG) and to generate enzimatically active plasmin (PLA) on the bacteria surface. PLA-coated<jats:italic>Leptospira<\/jats:italic>can degrade immobilized ECM molecules, an activity with implications in host tissue penetration. Moreover, we have identified and characterized several proteins that may act as PLG-binding receptors, each of them competent to generate active plasmin. The PLA activity associated to the outer surface of<jats:italic>Leptospira<\/jats:italic>could hamper the host immune attack by conferring the bacteria some benefit during infection. The PLA-coated leptospires obstruct complement C3b and IgG depositions on the bacterial surface, most probably through degradation. The decrease of leptospiral opsonization might be an important aspect of the immune evasion strategy. We believe that the presence of PLA on the leptospiral surface may (i) facilitate host tissue penetration, (ii) help the bacteria to evade the immune system and, as a consequence, (iii) permit<jats:italic>Leptospira<\/jats:italic>to reach secondary sites of infection.<\/jats:p>","DOI":"10.1155\/2012\/758513","type":"journal-article","created":{"date-parts":[[2012,10,16]],"date-time":"2012-10-16T09:05:04Z","timestamp":1350378304000},"page":"1-17","source":"Crossref","is-referenced-by-count":38,"title":["Plasminogen Binding Proteins and Plasmin Generation on the Surface of<i>Leptospira<\/i>spp.: The Contribution to the Bacteria-Host Interactions"],"prefix":"10.1155","volume":"2012","author":[{"given":"Monica L.","family":"Vieira","sequence":"first","affiliation":[{"name":"Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil, 1500, 05503-900 S\u00e3o Paulo, SP, Brazil"},{"name":"Interunidades em Biotecnologia, Instituto de Ci\u00eancias Biom\u00e9dicas, USP, Avenida Prof. Lineu Prestes, 1730, 05508-900 S\u00e3o Paulo SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina V.","family":"Atzingen","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil, 1500, 05503-900 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosane","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil, 1500, 05503-900 S\u00e3o Paulo, SP, Brazil"},{"name":"Interunidades em Biotecnologia, Instituto de Ci\u00eancias Biom\u00e9dicas, USP, Avenida Prof. Lineu Prestes, 1730, 05508-900 S\u00e3o Paulo SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata S.","family":"Mendes","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil, 1500, 05503-900 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renan F.","family":"Domingos","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil, 1500, 05503-900 S\u00e3o Paulo, SP, Brazil"},{"name":"Interunidades em Biotecnologia, Instituto de Ci\u00eancias Biom\u00e9dicas, USP, Avenida Prof. Lineu Prestes, 1730, 05508-900 S\u00e3o Paulo SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvio A.","family":"Vasconcellos","sequence":"additional","affiliation":[{"name":"Laboratorio de Zoonoses Bacterianas do VPS, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, USP, Avenida Prof. Dr. Orlando Marques de Paiva, 87, 05508-270 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana L. T. O.","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil, 1500, 05503-900 S\u00e3o Paulo, SP, Brazil"},{"name":"Interunidades em Biotecnologia, Instituto de Ci\u00eancias Biom\u00e9dicas, USP, Avenida Prof. Lineu Prestes, 1730, 05508-900 S\u00e3o Paulo SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","year":"1999"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.2.296-326.2001"},{"issue":"5","key":"3","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1097\/00001432-200110000-00005","volume":"14","year":"2001","journal-title":"Current Opinion in Infectious Diseases"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00830-2"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)80012-9"},{"issue":"9","key":"6","doi-asserted-by":"crossref","first-page":"e40","DOI":"10.1086\/339942","volume":"34","year":"2002","journal-title":"Clinical Infectious Diseases"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)01280-6"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/nature01597"},{"issue":"4","key":"9","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1590\/S0100-879X2004000400003","volume":"37","year":"2004","journal-title":"Brazilian Journal of Medical and Biological Research"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.7.2164-2172.2004"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0603979103"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001607"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03619.x"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030097"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-70"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.219931"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(03)00099-1"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00460-06"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.011916-0"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01635-06"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001188"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01639-07"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01643-07"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2009.07.010"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.03.050"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2009.10.047"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6445(01)00067-5"},{"issue":"7","key":"28","doi-asserted-by":"crossref","first-page":"2478","DOI":"10.1128\/IAI.63.7.2478-2484.1995","volume":"63","year":"1995","journal-title":"Infection and Immunity"},{"issue":"5","key":"29","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1093\/infdis\/171.5.1258","volume":"171","year":"1995","journal-title":"Journal of Infectious Diseases"},{"issue":"9","key":"30","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1128\/IAI.63.9.3491-3496.1995","volume":"63","year":"1995","journal-title":"Infection and Immunity"},{"issue":"7","key":"31","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1016\/S0092-8674(00)80298-6","volume":"89","year":"1997","journal-title":"Cell"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2143(99)90095-1"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2000.0984"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.9.5832-5839.2001"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00717.x"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00353-09"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011259"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021962"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05583-11"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-50"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2011.06.008"},{"issue":"4","key":"42","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/0035-9203(70)90087-8","volume":"64","year":"1970","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"issue":"8","key":"43","doi-asserted-by":"crossref","first-page":"3929","DOI":"10.1128\/IAI.67.8.3929-3936.1999","volume":"67","year":"1999","journal-title":"Infection and Immunity"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(98)01336-X"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340110203"},{"key":"46","doi-asserted-by":"crossref","first-page":"D257","DOI":"10.1093\/nar\/gkj079","volume":"34","year":"2006","journal-title":"Nucleic Acids Research"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.11.5857"},{"key":"48","doi-asserted-by":"crossref","first-page":"D247","DOI":"10.1093\/nar\/gkj149","volume":"34","year":"2006","journal-title":"Nucleic Acids Research"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1110\/ps.0303703"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01133-08"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28787-0"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.004531"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.2174\/1874285800903010069"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2005.07.003"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.9.4936-4945.2002"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.4.2276-2285.2000"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.5.3110"},{"issue":"3","key":"58","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1093\/infdis\/164.3.515","volume":"164","year":"1991","journal-title":"Journal of Infectious Diseases"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.tb20005.x"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.35.22346"},{"key":"61","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8652(199611)53:3<151::AID-AJH1>3.0.CO;2-Y"},{"issue":"5084","key":"62","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1126\/science.1439793","volume":"258","year":"1992","journal-title":"Science"},{"issue":"3 i","key":"63","first-page":"1485","volume":"78","year":"1981","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"64","doi-asserted-by":"publisher","DOI":"10.1086\/314654"},{"issue":"6","key":"65","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1172\/JCI106927","volume":"51","year":"1972","journal-title":"Journal of Clinical Investigation"},{"key":"66","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.12.6926-6932.2002"},{"key":"67","doi-asserted-by":"publisher","DOI":"10.1038\/nature08184"},{"key":"69","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.5.2311-2318.2002"},{"key":"70","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00741-06"},{"key":"71","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.038307-0"},{"key":"72","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00221-10"},{"issue":"16","key":"83","doi-asserted-by":"crossref","first-page":"5204","DOI":"10.1128\/jb.174.16.5204-5210.1992","volume":"174","year":"1992","journal-title":"Journal of Bacteriology"},{"key":"74","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027502"},{"key":"75","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.04.025"},{"key":"76","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1824"},{"key":"77","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2007.06.149"},{"key":"78","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2006.06.011"},{"key":"79","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.02.004"},{"key":"80","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2008.08.004"},{"key":"81","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-007-0273-2"},{"key":"82","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00214-08"}],"container-title":["Journal of Biomedicine and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2012\/758513.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2012\/758513.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2012\/758513.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T20:18:21Z","timestamp":1594757901000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2012\/758513\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012]]},"references-count":81,"alternative-id":["758513","758513"],"URL":"https:\/\/doi.org\/10.1155\/2012\/758513","relation":{},"ISSN":["1110-7243","1110-7251"],"issn-type":[{"value":"1110-7243","type":"print"},{"value":"1110-7251","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012]]}}}