{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T15:20:28Z","timestamp":1746804028691,"version":"3.40.5"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2012,3,14]],"date-time":"2012-03-14T00:00:00Z","timestamp":1331683200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Peptides"],"published-print":{"date-parts":[[2012,3,14]]},"abstract":"<jats:p>Antimicrobial peptides (AMP\u2019s) protect epithelial surfaces including epididymis against pathogens and play a key role in orchestrating various defensive responses. Recently, we have identified one such AMP, rabbit epididymal hemoglobin-\u03b2 subuit (REHb\u03b2P) from the epididymal fluid of rabbit,<jats:italic>Oryctologus cuniculus<\/jats:italic>. The demonstration of a protective role of REHb\u03b2P in epididymal epithelial cells (EPEC\u2019s) led us to investigate: (1) the identification of LPS interactive domain in REHb\u03b2P, and (2) whether the REHb\u03b2P of rabbit origin mediates vaginal cellular immune responses of another species (human). HeLa-S3, human vaginal epithelial cells (hVECs) were exposed to LPS or the LPS-stimulated cells treated with REHb\u03b2P or neutral peptide,<jats:italic>n<\/jats:italic>REHb\u03b2P. Effect of LPS and cytokines (IL-6 and IL-1<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mi>\u03b1<\/mml:mi><\/mml:math>) and chemokines (IL-8, MCP-1) levels was determined in the culture supernatants. In response to the LPS, hVECs synthesized these mediators and the levels were significantly higher than controls. This enhancing effect was ameliorated when the LPS-induced hVECs were treated with REHb\u03b2P. Similar results were obtained on NF-\u03baB protein and hBD-1 mRNA expression. Confocal microscopy studies revealed that REHb\u03b2P attenuated the LPS-induced internalization of<jats:italic>E. coli<\/jats:italic>by macrophages. The chemotaxis studies performed using Boyden chamber Transwell assay, which showed elevated migration of U937 cells when the supernatants of LPS-induced hVECs were used, and the effect was inhibited by REHb\u03b2P. REHb\u03b2P was found to be localized on the acrosome of rabbit spermatozoa, suggesting its role in sperm protection beside sperm function. In conclusion, REHb\u03b2P may have the potential to develop as a therapeutic agent for reproductive tract infections (RTI\u2019s).<\/jats:p>","DOI":"10.1155\/2012\/782019","type":"journal-article","created":{"date-parts":[[2012,3,14]],"date-time":"2012-03-14T21:02:39Z","timestamp":1331758959000},"page":"1-10","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Rabbit Epididymal Antimicrobial Peptide, REHb<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mi mathvariant=\"bold\">\u03b2<\/mml:mi><\/mml:math>P, on LPS-Induced Proinflammatory Cytokine Responses in Human Vaginal Cells<i>In Vitro<\/i>"],"prefix":"10.1155","volume":"2012","author":[{"given":"K. V. R.","family":"Reddy","sequence":"first","affiliation":[{"name":"Division of Molecular Immunology, National Institute for Research in Reproductive Health (NIRRH), Indian Council of Medical Research (ICMR), Jehangir Merwanji Street, Parel, Mumbai 400 012, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Sukanya","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology, National Institute for Research in Reproductive Health (NIRRH), Indian Council of Medical Research (ICMR), Jehangir Merwanji Street, Parel, Mumbai 400 012, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. S.","family":"Patgaonkar","sequence":"additional","affiliation":[{"name":"Division of Molecular Immunology, National Institute for Research in Reproductive Health (NIRRH), Indian Council of Medical Research (ICMR), Jehangir Merwanji Street, Parel, Mumbai 400 012, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Selvaakumar","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioinformatics, Padmashree Dr. D.Y. Patil University, CBD Belapur, Navi Mumbai 400 614, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.65.2.208-231.2001"},{"issue":"3\u20135","key":"2","first-page":"169","volume":"187","year":"1993","journal-title":"Immunobiology"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1177\/09680519010070030101"},{"issue":"6","key":"4","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1093\/oxfordjournals.jbchem.a135218","volume":"97","year":"1985","journal-title":"The Journal of Biochemistry"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki350"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.082180"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2005.00687.x"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.036426"},{"issue":"5","key":"9","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1002\/j.1939-4640.2002.tb02295.x","volume":"23","year":"2002","journal-title":"Journal of Andrology"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.031567"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1126\/science.1056545"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.102.004564"},{"issue":"5","key":"13","first-page":"2516","volume":"169","year":"2010","journal-title":"The Journal of Immunology"},{"issue":"7","key":"14","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1093\/molehr\/7.7.625","volume":"7","year":"2001","journal-title":"Molecular Human Reproduction"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4297-4302.2000"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1210\/en.142.10.4529"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2010.10.012"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(03)00245-9"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.380"},{"key":"20","first-page":"6859","volume":"13","year":"2008","journal-title":"Frontiers in Bioscience"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(03)00245-9"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.4172\/1948-5948.S2-002"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150181505"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340170404"},{"issue":"2","key":"25","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1002\/(SICI)1097-0134(20000501)39:2<178::AID-PROT8>3.0.CO;2-6","volume":"39","year":"2000","journal-title":"Proteins"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.7.3901-3908.2003"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2009.05.009"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2008.04.005"},{"issue":"1-2","key":"29","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","year":"1976","journal-title":"Analytical Biochemistry"},{"issue":"3-4","key":"30","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.micpath.2010.12.007","volume":"50","year":"2011","journal-title":"Microbial Pathogenesis"},{"key":"31","first-page":"107","volume-title":"Measurement of nonopsonic phagocytic killing by human and mouse phagocytes","volume":"236","year":"1994"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.15.11058"},{"issue":"1","key":"33","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1165\/ajrcmb.26.1.4657","volume":"26","year":"2002","journal-title":"American Journal of Respiratory Cell and Molecular Biology"}],"container-title":["International Journal of Peptides"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/archive\/2012\/782019.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/archive\/2012\/782019.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/archive\/2012\/782019.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,21]],"date-time":"2024-04-21T12:15:06Z","timestamp":1713701706000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ijpep\/2012\/782019\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,14]]},"references-count":33,"alternative-id":["782019","782019"],"URL":"https:\/\/doi.org\/10.1155\/2012\/782019","relation":{},"ISSN":["1687-9767","1687-9775"],"issn-type":[{"type":"print","value":"1687-9767"},{"type":"electronic","value":"1687-9775"}],"subject":[],"published":{"date-parts":[[2012,3,14]]}}}