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To shed light on the effect of HPV on upstream receptors, we evaluated TLRs 1\u20139 gene expression in HPV-negative normal and HPV-positive pre-malignant and malignant<jats:italic>ex vivo<\/jats:italic>cervical tissue. Quantitative real-time polymerase chain reaction was performed separately for epithelial and stromal tissue compartments. Differences in gene expression were analyzed by the Jonckheere-Terpstra trend test or the Student's<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mi>t<\/mml:mi><\/mml:math>-test for pairwise comparison. Laser capture microdissection revealed an increase in TLR3 and a decrease in TLR1 mRNA levels in dysplastic and carcinoma epithelium, respectively. In the stroma, a trend of increasing TLR 1, 2, 5, 6, and 9 mRNA levels with disease severity was found. These findings implicate the involvement of TLR3 and TLR1 in early and late cervical carcinogenesis, respectively, suggesting that stromal upregulation of TLRs may play a role in cervical disease progression.<\/jats:p>","DOI":"10.1155\/2012\/785825","type":"journal-article","created":{"date-parts":[[2011,10,13]],"date-time":"2011-10-13T15:04:28Z","timestamp":1318518268000},"page":"1-9","source":"Crossref","is-referenced-by-count":59,"title":["Toll-Like Receptor Transcriptome in the HPV-Positive Cervical Cancer Microenvironment"],"prefix":"10.1155","volume":"2012","author":[{"given":"Correne A.","family":"DeCarlo","sequence":"first","affiliation":[{"name":"Probe Development and Biomarker Exploration Thunder Bay Regional Research Institute, Thunder Bay, ON, Canada P7B 6V4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce","family":"Rosa","sequence":"additional","affiliation":[{"name":"Biorefining Research Initiative and Department of Biology, Lakehead University, Thunder Bay, ON, Canada P7B 5E1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Jackson","sequence":"additional","affiliation":[{"name":"Probe Development and Biomarker Exploration Thunder Bay Regional Research Institute, Thunder Bay, ON, Canada P7B 6V4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Niccoli","sequence":"additional","affiliation":[{"name":"Probe Development and Biomarker Exploration Thunder Bay Regional Research Institute, Thunder Bay, ON, Canada P7B 6V4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas G.","family":"Escott","sequence":"additional","affiliation":[{"name":"Thunder Bay Regional Health Sciences Centre, Department of Pathology, Thunder Bay, ON, Canada P7B 6V4"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingeborg","family":"Zehbe","sequence":"additional","affiliation":[{"name":"Probe Development and Biomarker Exploration Thunder Bay Regional Research Institute, Thunder Bay, ON, Canada P7B 6V4"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1007\/s12307-009-0022-y"},{"issue":"7","key":"2","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/nri1391","volume":"4","year":"2004","journal-title":"Nature Reviews Immunology"},{"issue":"927","key":"3","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1098\/rspb.1957.0048","volume":"147","year":"1957","journal-title":"Proceedings of the Royal Society of London. 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