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The model was validated experimentally and the maximum protease production was found 315.28 U using optimum culture conditions. The protease was purified using ammonium sulphate (60%) precipitation technique. The HPLC analysis of dialyzed sample showed that the retention time is 1.84\u2009min with 73.5% purity. This enzyme retained more than 92% of its initial activity after preincubation for 30\u2009min at <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\"><mml:mrow><mml:msup><mml:mrow><mml:mn>37<\/mml:mn><\/mml:mrow><mml:mo>\u2218<\/mml:mo><\/mml:msup><mml:mtext>C<\/mml:mtext><\/mml:mrow><\/mml:math>\n in the presence of 25%\u2009v\/v DMSO, methanol, ethanol, ACN, 2-propanol, benzene, toluene, and hexane. In addition, partially purified enzyme showed remarkable stability for 60\u2009min at room temperature, in the presence of anionic detergent (Tween-80 and Triton X-100), surfactant (SDS), bleaching agent (sodium perborate and hydrogen peroxide), and anti-redeposition agents (Na<jats:sub>2<\/jats:sub>CMC, Na<jats:sub>2<\/jats:sub>CO<jats:sub>3<\/jats:sub>). 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