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Chlorotoxin also binds to matrix metalloproteinase-2 and other proteins on glioma cell surfaces. Glioma cells are considered to require the activation of matrix metalloproteinase-2 during invasion and migration. In this study, for targeting glioma, we designed two types of recombinant chlorotoxin fused to human IgG-Fcs with\/without a hinge region. Chlorotoxin fused to IgG-Fcs was designed as a dimer of 60\u2009kDa with a hinge region and a monomer of 30\u2009kDa without a hinge region. The monomeric and dimeric forms of chlorotoxin inhibited cell proliferation at 300\u2009nM and induced internalization in human glioma A172 cells. The monomer had a greater inhibitory effect than the dimer; therefore, monomeric chlorotoxin fused to IgG-Fc was multivalently displayed on the surface of bionanocapsules to develop a drug delivery system that targeted matrix metalloproteinase-2. The target-dependent internalization of bionanocapsules in A172 cells was observed when chlorotoxin was displayed on the bionanocapsules. This study indicates that chlorotoxin fused to IgG-Fcs could be useful for the active targeting of glioblastoma cells.<\/jats:p>","DOI":"10.1155\/2012\/975763","type":"journal-article","created":{"date-parts":[[2012,12,5]],"date-time":"2012-12-05T23:57:23Z","timestamp":1354751843000},"page":"1-10","source":"Crossref","is-referenced-by-count":18,"title":["Chlorotoxin Fused to IgG-Fc Inhibits Glioblastoma Cell Motility via Receptor-Mediated Endocytosis"],"prefix":"10.1155","volume":"2012","author":[{"given":"Tomonari","family":"Kasai","sequence":"first","affiliation":[{"name":"Department of Medical and Bioengineering Science, Graduate School of Natural Science and Technology, Okayama University, Okayama 7008530, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Medical and Bioengineering Science, Graduate School of Natural Science and Technology, Okayama University, Okayama 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