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RTT, an X chromosome-linked disorder associated with mutations in<jats:italic>MECP2<\/jats:italic>, is the leading cause of intellectual disabilities in women. Neurons in<jats:italic>Mecp2<\/jats:italic>-deficient mice show lower dendritic spine density in several brain regions. To better understand the role of MeCP2 on excitatory spine synapses, we analyzed dendritic spines of CA1 pyramidal neurons in the hippocampus of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msup><mml:mrow><mml:mi>M<\/mml:mi><mml:mi>e<\/mml:mi><mml:mi>c<\/mml:mi><mml:mi>p<\/mml:mi><mml:mn>2<\/mml:mn><\/mml:mrow><mml:mrow><mml:mi>t<\/mml:mi><mml:mi>m<\/mml:mi><mml:mn>1.1<\/mml:mn><mml:mtext>Jae<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>male mutant mice by either confocal microscopy or electron microscopy (EM). At postnatal-day 7 (P7), well before the onset of RTT-like symptoms, CA1 pyramidal neurons from mutant mice showed lower dendritic spine density than those from wildtype littermates. On the other hand, at P15 or later showing characteristic RTT-like symptoms, dendritic spine density did not differ between mutant and wildtype neurons. Consistently, stereological analyses at the EM level revealed similar densities of asymmetric spine synapses in CA1<jats:italic>stratum radiatum<\/jats:italic>of symptomatic mutant and wildtype littermates. These results raise caution regarding the use of dendritic spine density in hippocampal neurons as a phenotypic endpoint for the evaluation of therapeutic interventions in symptomatic<jats:italic>Mecp2<\/jats:italic>-deficient mice. However, they underscore the potential role of MeCP2 in the maintenance of excitatory spine synapses.<\/jats:p>","DOI":"10.1155\/2012\/976164","type":"journal-article","created":{"date-parts":[[2012,7,30]],"date-time":"2012-07-30T17:01:26Z","timestamp":1343667686000},"page":"1-9","source":"Crossref","is-referenced-by-count":33,"title":["Hippocampal CA1 Pyramidal Neurons of<i>Mecp2<\/i>Mutant Mice Show a Dendritic Spine Phenotype Only in the Presymptomatic Stage"],"prefix":"10.1155","volume":"2012","author":[{"given":"Christopher A.","family":"Chapleau","sequence":"first","affiliation":[{"name":"Department of Neurobiology, Civitan International Research Center, The University of Alabama at Birmingham, Birmingham, AL 35294, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena Maria","family":"Boggio","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, University of Torino and National Institute of Neuroscience, Turin 10126, 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