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Its protective effect has been associated with its ability to induce cytoprotective enzymes through an Nrf2-dependent pathway. Gentamicin (GM) is a widely used antibiotic; nephrotoxicity is the main side effect of this compound. In this study, it was investigated if SFN is able to induce protection against GM-induced nephropathy both in renal epithelial LLC-PK1 cells in culture and in rats. SFN prevented GM-induced death and loss of mitochondrial membrane potential in LLC-PK1 cells. In addition, it attenuated GM-induced renal injury (proteinuria, increases in serum creatinine, in blood urea nitrogen, and in urinary excretion on N-acetyl-<jats:italic>\u03b2<\/jats:italic>-D-glucosaminidase, and decrease in creatinine clearance and in plasma glutathione peroxidase activity) and necrosis and apoptosis in rats. The apoptotic death was associated with enhanced active caspase-9. Caspase-8 was unchanged in all the studied groups. In addition, SFN was able to prevent GM-induced protein nitration and decrease in the activity of antioxidant enzymes catalase and glutathione peroxidase in renal cortex. In conclusion, the protective effect of SFN against GM-induced acute kidney injury could be associated with the preservation in mitochondrial function that would prevent the intrinsic apoptosis and nitrosative stress.<\/jats:p>","DOI":"10.1155\/2013\/135314","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T18:28:09Z","timestamp":1365100089000},"page":"1-17","source":"Crossref","is-referenced-by-count":29,"title":["Sulforaphane Attenuates Gentamicin-Induced Nephrotoxicity: Role of Mitochondrial Protection"],"prefix":"10.1155","volume":"2013","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2370-1841","authenticated-orcid":true,"given":"Mario","family":"Negrette-Guzm\u00e1n","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda, Facultad de Qu\u00edmica, UNAM, 04510 Mexico City, DF, 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