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We studied also the effects of HHC and HHDC on possible molecular mechanisms which participate in the activation of NADPH oxidase, that is, on PKC activity, on phosphorylation of some PKC isoforms (<jats:italic>\u03b1<\/jats:italic>,<jats:italic>\u03b2<\/jats:italic>II, and<jats:italic>\u03b4<\/jats:italic>), and on phosphorylation of the NADPH oxidase subunit p40<jats:sup><jats:italic>phox<\/jats:italic><\/jats:sup>. Without affecting cytotoxicity, both coumarines tested were effective inhibitors\/scavengers of ROS produced by neutrophils on extracellular level. HHC markedly diminished oxidant production and also, intracellularly, decreased PKC activity and partly phosphorylation of PKC<jats:italic>\u03b1<\/jats:italic>,<jats:italic>\u03b2<\/jats:italic>II. On the other hand, we did not observe any effect of coumarin derivatives on phosphorylation of PKC<jats:italic>\u03b4<\/jats:italic>and on phosphorylation of the NADPH oxidase subunit p40<jats:sup><jats:italic>phox<\/jats:italic><\/jats:sup>, which were suggested to be involved in the PMA-dependent intracellular activation process. In agreement with our previous findings, we assume that the different molecular structures of HHC and HHDC with their different physicochemical and free radical scavenging characteristics are responsible for their diverse effects on the parameters tested.<\/jats:p>","DOI":"10.1155\/2013\/136570","type":"journal-article","created":{"date-parts":[[2013,11,17]],"date-time":"2013-11-17T21:02:07Z","timestamp":1384722127000},"page":"1-10","source":"Crossref","is-referenced-by-count":10,"title":["Study of Possible Mechanisms Involved in the Inhibitory Effects of Coumarin Derivatives on Neutrophil Activity"],"prefix":"10.1155","volume":"2013","author":[{"given":"Katar\u00edna","family":"Dr\u00e1bikov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Experimental Pharmacology and Toxicology Slovak Academy of Sciences, D\u00fabravsk\u00e1 cesta 9, 841 04 Bratislava, 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