{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T15:48:27Z","timestamp":1774453707518,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,12,18]],"date-time":"2013-12-18T00:00:00Z","timestamp":1387324800000},"content-version":"vor","delay-in-days":351,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p>Brucellosis is a reemerging infectious zoonotic disease of worldwide importance. In human, it is mainly caused by <jats:italic>Brucella melitensis<\/jats:italic>, a natural pathogen for goats. In India, a large number of goats are reared in semi\u2010intensive to intensive system within the close vicinity of human being. At present, there is no vaccination and control strategy for caprine brucellosis in the country. Thus, to formulate an effective control strategy, the status of etiological agent is essential. To cope up with these, the present study was conducted to isolate and identify the prevalent <jats:italic>Brucella<\/jats:italic> species in caprine brucellosis in India. The 30 samples (fetal membrane, fetal stomach content and vaginal swabs) collected throughout India from the aborted fetus of goats revealed the isolation of 05 isolates all belonging to <jats:italic>Brucella melitensis<\/jats:italic> biovars 3. All the isolates produced amplification products of 1412 and 720\u2009bp in polymerase chain reaction with genus and species specific 16S rRNA and omp31 gene based primers, respectively. Moreover, the amplification of omp31 gene in all the isolates confirmed the presence of immuno dominant outer membrane protein (31\u2009kDa omp) in all the field isolates of <jats:italic>B. melitensis<\/jats:italic> in aborted foetus of goats in India. These findings can support the development of omp31 based specific serodiagnostic test as well as vaccine for the control of caprine brucellosis in India.<\/jats:p>","DOI":"10.1155\/2013\/160376","type":"journal-article","created":{"date-parts":[[2013,12,18]],"date-time":"2013-12-18T21:54:36Z","timestamp":1387403676000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["16S rRNA and Omp31 Gene Based Molecular Characterization of Field Strains of <i>B. melitensis<\/i> from Aborted Foetus of Goats in India"],"prefix":"10.1155","volume":"2013","author":[{"given":"Ajay","family":"Singh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivek Kumar","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vikas Kumar","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shivasharanappa","family":"Nayakwadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,12,18]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-008-0072-1"},{"key":"e_1_2_8_2_2","first-page":"3","article-title":"Brucellosis: an updated review of the disease","volume":"83","author":"Manish K.","year":"2013","journal-title":"Indian Journal of Animal Sciences"},{"key":"e_1_2_8_3_2","unstructured":"SCAHAW the European\u2019s Scientific Committee on Animal Health and Animal Welfare Brucellosis in sheep and Goats (Brucella melitensis) A report of the Scientific Committee on Animal Health and Animal Welfare European Commission 2001."},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70382-6"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.12.023"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2005003"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.65356-0"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.11.2660-2666.1994"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-25-1-135"},{"key":"e_1_2_8_10_2","first-page":"377","volume-title":"Bergey\u2032s Manual of Systematic Bacteriology","author":"Corbel M. 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