{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:39:08Z","timestamp":1792175948073,"version":"4.4.0"},"reference-count":32,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Programa de Mejoramiento del Profesorado (PROMEP)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p><jats:italic>Bacillus thuringiensis<\/jats:italic>is a gram-positive soil-dwelling bacterium that is commonly used as a biological pesticide. This bacterium may also be used for biological control of helminth parasites in domestic animals. In this study, we evaluated the possible ovicidal and cestocidal effects of a total protein extract of<jats:italic>B. thuringiensis<\/jats:italic>native strains on the zoonotic cestode parasite of dogs,<jats:italic>Dipylidium caninum<\/jats:italic>(<jats:italic>D. caninum<\/jats:italic>). Dose and time response curves were determined by coincubating<jats:italic>B. thuringiensis<\/jats:italic>proteins at concentration ranging from 100 to 1000\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL along with 4000 egg capsules of<jats:italic>D. caninum<\/jats:italic>. Egg viability was evaluated using the trypan blue exclusion test. The lethal concentration of toxins on eggs was 600\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml, and the best incubation time to produce this effect was 3\u2009h. In the adult stage, the motility and the thickness of the tegument were used as indicators of damage. The motility was inhibited by 100% after 8 hours of culture compared to the control group, while the thickness of the cestode was reduced by 34%. Conclusively, proteins of the strain GP526 of<jats:italic>B. thuringiensis<\/jats:italic>directly act upon<jats:italic>D. caninum<\/jats:italic>showing ovicidal and cestocidal effects. Thus,<jats:italic>B. thuringiensis<\/jats:italic>is proposed as a potential biological control agent against this zoonosis.<\/jats:p>","DOI":"10.1155\/2013\/174619","type":"journal-article","created":{"date-parts":[[2012,12,29]],"date-time":"2012-12-29T21:00:50Z","timestamp":1356814850000},"page":"1-7","source":"Crossref","is-referenced-by-count":8,"title":["<i>In Vitro<\/i>Ovicidal and Cestocidal Effects of Toxins from<i>Bacillus thuringiensis<\/i>on the Canine and Human Parasite<i>Dipylidium caninum<\/i>"],"prefix":"10.1155","volume":"2013","author":[{"given":"Guadalupe","family":"Pe\u00f1a","sequence":"first","affiliation":[{"name":"Centro de Investigaciones Biol\u00f3gicas, Universidad Aut\u00f3noma del Estado de Morelos, Avenida Universidad 1001, Col. 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