{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T15:38:36Z","timestamp":1740152316485,"version":"3.37.3"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,2,20]],"date-time":"2013-02-20T00:00:00Z","timestamp":1361318400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100010884","name":"Carl M. Hansen Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010884","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Washington State Agricultural Research Center"},{"name":"College of Veterinary Medicine Agricultural Animal Health"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Advances in Bioinformatics"],"published-print":{"date-parts":[[2013,2,20]]},"abstract":"<jats:p>In this paper we present a new ab initio approach for constructing an unrooted dendrogram using protein clusters, an approach that has the potential for estimating relationships among several thousands of species based on their putative proteomes. We employ an open-source software program called<jats:italic>pClust<\/jats:italic>that was developed for use in metagenomic studies. Sequence alignment is performed by<jats:italic>pClust<\/jats:italic>using the Smith-Waterman algorithm, which is known to give optimal alignment and, hence, greater accuracy than BLAST-based methods. Protein clusters generated by<jats:italic>pClust<\/jats:italic>are used to create protein profiles for each species in the dendrogram, these profiles forming a correlation filter library for use with a new taxon. To augment the dendrogram with a new taxon, a protein profile for the taxon is created using BLASTp, and this new taxon is placed into a position within the dendrogram corresponding to the highest correlation with profiles in the correlation filter library. This work was initiated because of our interest in plasmids, and each step is illustrated using proteomes from Gram-negative bacterial plasmids. Proteomes for 527 plasmids were used to generate the dendrogram, and to demonstrate the utility of the insertion algorithm twelve recently sequenced pAKD plasmids were used to augment the dendrogram.<\/jats:p>","DOI":"10.1155\/2013\/191586","type":"journal-article","created":{"date-parts":[[2013,2,20]],"date-time":"2013-02-20T22:34:21Z","timestamp":1361399661000},"page":"1-8","source":"Crossref","is-referenced-by-count":9,"title":["Using Protein Clusters from Whole Proteomes to Construct and Augment a Dendrogram"],"prefix":"10.1155","volume":"2013","author":[{"given":"Yunyun","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Electrical Engineering and Computer Science, Washington State University, P.O. 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