{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:29:52Z","timestamp":1788870592525,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81172612"],"award-info":[{"award-number":["81172612"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["12Y03001"],"award-info":[{"award-number":["12Y03001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004605","name":"Zhengzhou University","doi-asserted-by":"publisher","award":["81172612"],"award-info":[{"award-number":["81172612"]}],"id":[{"id":"10.13039\/501100004605","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004605","name":"Zhengzhou University","doi-asserted-by":"publisher","award":["12Y03001"],"award-info":[{"award-number":["12Y03001"]}],"id":[{"id":"10.13039\/501100004605","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>\n                    <jats:italic>Spirometra erinaceieuropaei<\/jats:italic>\n                    cysteine protease (SeCP) in sparganum ES proteins recognized by early infection sera was identified by MALDI-TOF\/TOF-MS. The aim of this study was to predict the structures and functions of SeCP protein by using the full length cDNA sequence of SeCP gene with online sites and software programs. The SeCP gene sequence was of 1 053\u2009bp length with a 1011\u2009bp biggest ORF encoding 336-amino acid protein with a complete cathepsin propeptide inhibitor domain and a peptidase C1A conserved domain. The predicted molecular weight and isoelectric point of SeCP were 37.87\u2009kDa and 6.47, respectively. The SeCP has a signal peptide site and no transmembrane domain, located outside the membrane. The secondary structure of SeCP contained 8\n                    <jats:italic>\u03b1<\/jats:italic>\n                    -helixes, 7\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -strands, and 20 coils. The SeCP had 15 potential antigenic epitopes and 19 HLA-I restricted epitopes. Based on the phylogenetic analysis of SeCP,\n                    <jats:italic>S. erinaceieuropaei<\/jats:italic>\n                    has the closest evolutionary status with\n                    <jats:italic>S. mansonoides<\/jats:italic>\n                    . SeCP was a kind of proteolytic enzyme with a variety of biological functions and its antigenic epitopes could provide important insights on the diagnostic antigens and target molecular of antisparganum drugs.\n                  <\/jats:p>","DOI":"10.1155\/2013\/198250","type":"journal-article","created":{"date-parts":[[2013,12,12]],"date-time":"2013-12-12T16:02:02Z","timestamp":1386864122000},"page":"1-7","source":"Crossref","is-referenced-by-count":10,"title":["Analysis of Structures, Functions, and Epitopes of Cysteine Protease from\n                    <i>Spirometra erinaceieuropaei<\/i>\n                    Spargana"],"prefix":"10.1155","volume":"2013","author":[{"given":"Li Na","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong Quan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","year":"2005"},{"key":"2","year":"2009"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.09.018"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2007.12.003"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(99)01405-2"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2009.07.001"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2011.03.011"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e3181c91a15"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.3201\/eid1701.101095"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.surneu.2006.06.056"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(94)90218-6"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-2206-2"},{"issue":"3","key":"13","first-page":"408","volume":"8","year":"2013","journal-title":"Iranian Journal of Parasitology"},{"issue":"3","key":"14","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.sbi.2009.03.008","volume":"19","year":"2009","journal-title":"Current Opinion in Structural Biology"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq427"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-9-40"},{"issue":"4","key":"17","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1038\/nprot.2010.5","volume":"5","year":"2010","journal-title":"Nature Protocols"},{"key":"18","doi-asserted-by":"crossref","first-page":"W471","DOI":"10.1093\/nar\/gks372","volume":"40","year":"2012","journal-title":"Nucleic Acids Research"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/0263-7855(96)00018-5"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg520"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900140"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msm092"},{"issue":"4","key":"23","first-page":"406","volume":"4","year":"1987","journal-title":"Molecular Biology and Evolution"},{"issue":"6","key":"24","doi-asserted-by":"crossref","first-page":"2120","DOI":"10.1128\/IAI.63.6.2120-2125.1995","volume":"63","year":"1995","journal-title":"Infection and Immunity"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(96)02703-X"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0896(99)00004-8"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.2307\/3283911"},{"key":"28","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S0065-308X(08)60243-2","volume":"43","year":"1999","journal-title":"Advances in Parasitology"},{"issue":"5","key":"29","first-page":"351","volume":"35","year":"1985","journal-title":"Japanese Journal of Parasitology"},{"issue":"3","key":"30","first-page":"191","volume":"30","year":"1992","journal-title":"Kisaengchunghak Chapchi"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2008.46.3.183"},{"issue":"4","key":"32","doi-asserted-by":"crossref","first-page":"517","DOI":"10.2307\/3283376","volume":"79","year":"1993","journal-title":"Journal of Parasitology"},{"key":"33","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1017\/S0031182000076496","volume":"109","year":"1994","journal-title":"Parasitology"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/198250.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/198250.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/198250.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,11]],"date-time":"2020-08-11T21:12:15Z","timestamp":1597180335000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2013\/198250\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":33,"alternative-id":["198250","198250"],"URL":"https:\/\/doi.org\/10.1155\/2013\/198250","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013]]}}}