{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T17:51:41Z","timestamp":1740160301627,"version":"3.37.3"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology","doi-asserted-by":"publisher","award":["24790367"],"award-info":[{"award-number":["24790367"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Hepatology"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>.<jats:italic>In situ<\/jats:italic>hybridization (ISH) with high sensitivity has been requested to demonstrate hepatitis C virus (HCV) RNA in formalin-fixed, paraffin-embedded (FFPE) sections of the liver.<jats:italic>Methods<\/jats:italic>. ISH employing a locked-nucleic-acid- (LNA-)modified oligonucleotide probe and biotin-free catalyzed signal amplification system (CSAII) was applied to HCV-RNA detection in the liver tissue. Nested reverse-transcription polymerase chain reaction (RT-PCR) was performed for HCV genotyping using total RNA extracted from FFPE sections. The target tissues included FFPE tissue sections of humanized livers in HCV-infected chimeric mice (HCV genotypes 1a, 1b, and 2a and noninfected) and of needle-biopsied livers from HCV-infected patients.<jats:italic>Results<\/jats:italic>. HCV-RNA was demonstrated with the ISH technique in HCV-infected liver tissues from both chimeric mice and 9 (82%) of 11 patients with HCV infection. The HCV signals were sensitive to RNase. Nested RT-PCR confirmed the genotype in 8 (73%) of 11 livers (type 1b: 6 lesions and type 2a: 2 lesions). HCV-RNA was not identified in chronic hepatitis B lesions, fatty liver, autoimmune hepatitis, and hepatocellular carcinoma.<jats:italic>Conclusion<\/jats:italic>. ISH using the LNA-modified oligonucleotide probe and CSAII was applicable to detecting HCV-RNA in routinely prepared FFPE liver specimens.<\/jats:p>","DOI":"10.1155\/2013\/249535","type":"journal-article","created":{"date-parts":[[2013,6,18]],"date-time":"2013-06-18T21:01:11Z","timestamp":1371589271000},"page":"1-7","source":"Crossref","is-referenced-by-count":2,"title":["Demonstration of Hepatitis C Virus RNA with<i>In Situ<\/i>Hybridization Employing a Locked Nucleic Acid Probe in Humanized Liver of Infected Chimeric Mice and in Needle-Biopsied Human Liver"],"prefix":"10.1155","volume":"2013","author":[{"given":"Kazuya","family":"Shiogama","sequence":"first","affiliation":[{"name":"Department of Pathology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken-ichi","family":"Inada","sequence":"additional","affiliation":[{"name":"Department of Pathology, Fujita Health University School of 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