{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T13:13:59Z","timestamp":1773321239150,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,4,29]],"date-time":"2013-04-29T00:00:00Z","timestamp":1367193600000},"content-version":"vor","delay-in-days":118,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"},{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2007CB947704"],"award-info":[{"award-number":["2007CB947704"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2006CB500700"],"award-info":[{"award-number":["2006CB500700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["Z0005187040311"],"award-info":[{"award-number":["Z0005187040311"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2007CB947704"],"award-info":[{"award-number":["2007CB947704"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2006CB500700"],"award-info":[{"award-number":["2006CB500700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["Z0005187040311"],"award-info":[{"award-number":["Z0005187040311"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Beijing Committee of Science and Technology","award":["2007CB947704"],"award-info":[{"award-number":["2007CB947704"]}]},{"name":"Beijing Committee of Science and Technology","award":["2006CB500700"],"award-info":[{"award-number":["2006CB500700"]}]},{"name":"Beijing Committee of Science and Technology","award":["Z0005187040311"],"award-info":[{"award-number":["Z0005187040311"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neural Plasticity"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p>\n                    The aim of the present study is to detect a combination method to utilize gene therapy for the treatment of Parkinson\u2019s disease (PD). Here, a PD rat model is used for the\n                    <jats:italic>in vivo<\/jats:italic>\n                    gene therapy of a recombinant adeno\u2010associated virus (AAV2) containing a human glutamic acid decarboxylase 65 (rAAV2\u2010hGAD65) gene delivered to the subthalamic nucleus (STN). This is combined with the\n                    <jats:italic>ex vivo<\/jats:italic>\n                    gene delivery of tyrosine hydroxylase (TH) by fibroblasts injected into the striatum. After the treatment, the rotation behavior was improved with the greatest efficacy in the combination group. The results of immunohistochemistry showed that hGAD65 gene delivery by AAV2 successfully led to phenotypic changes of neurons in STN. And the levels of glutamic acid and GABA in the internal segment of the globus pallidus (GPi) and substantia nigra pars reticulata (SNr) were obviously lower than the control groups. However, hGAD65 gene transfer did not effectively protect surviving dopaminergic neurons in the SNc and VTA. This study suggests that subthalamic hGAD65 gene therapy and combined with TH gene therapy can alleviate symptoms of the PD model rats, independent of the protection the DA neurons from death.\n                  <\/jats:p>","DOI":"10.1155\/2013\/263287","type":"journal-article","created":{"date-parts":[[2013,4,29]],"date-time":"2013-04-29T17:02:28Z","timestamp":1367254948000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Subthalamic hGAD65 Gene Therapy and Striatum TH Gene Transfer in a Parkinson\u2019s Disease Rat Model"],"prefix":"10.1155","volume":"2013","author":[{"given":"Deyu","family":"Zheng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohua","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junpeng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deyi","family":"Duan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanying","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qunyuan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,4,29]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-14-04449.1996"},{"key":"e_1_2_7_2_2","first-page":"7","article-title":"Rationale for continuous dopaminomimetic therapy of Parkinson\u2032s disease","volume":"39","author":"Chase T. N.","year":"1989","journal-title":"Neurology"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2011.06.048"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00702-004-0251-7"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(73)90175-5"},{"key":"e_1_2_7_6_2","first-page":"1","article-title":"Levodopa in the treatment of Parkinson\u2032s disease","author":"Fahn S.","year":"2006","journal-title":"Journal of Neural Transmission"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90246-V"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresprot.2005.10.005"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1089\/10430340252792486"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3000350"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.266.5189.1399"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.21.12120"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.22.9011"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresprot.2005.03.002"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2006.04.008"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1089\/104303403321467180"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2004.15.1177"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.parkreldis.2010.06.008"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awg111"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.surneu.2007.05.058"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074549"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.16.8446-8454.2002"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1789.2006.00688.x"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1089\/rej.2009.0850"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.34.12.1566"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.19.8979"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00221-004-2075-y"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/376174a0"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/7449"},{"key":"e_1_2_7_30_2","first-page":"119","article-title":"Are GAD65 and GAD67 associated with specific pools of GABA in brain?","volume":"5","author":"Martin D. L.","year":"1998","journal-title":"Perspectives on Developmental Neurobiology"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1975.tb04409.x"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90077-D"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1980.tb06593.x"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2006.09.058"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410200106"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2396(199710)27:2<122::AID-SYN3>3.0.CO;2-G"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(97)00112-5"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2005.03.016"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-10-06256.1994"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1681.2003.03922.x"}],"container-title":["Neural Plasticity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2013\/263287","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/2013\/263287","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2013\/263287","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T15:38:00Z","timestamp":1769096280000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2013\/263287"}},"subtitle":[],"editor":[{"given":"Michel","family":"Baudry","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2013,1]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1155\/2013\/263287"],"URL":"https:\/\/doi.org\/10.1155\/2013\/263287","archive":["Portico"],"relation":{},"ISSN":["2090-5904","1687-5443"],"issn-type":[{"value":"2090-5904","type":"print"},{"value":"1687-5443","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1]]},"assertion":[{"value":"2012-12-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"263287"}}