{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:48:27Z","timestamp":1787017707961,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>\n                    Surgery is still the main treatment in hydatidosis caused by\n                    <jats:italic>Echinococcus<\/jats:italic>\n                    , which is a global health problem in human and animals. So, there is need for some natural protoscolicidal agents for instillation to prevent their reoccurrence at therapeutic doses. In this present investigation, anticestodal activity of one of the endophytic fungi\n                    <jats:italic>Pestalotiopsis<\/jats:italic>\n                    sp. from Neem plant was observed on protoscoleces of hydatid cysts of\n                    <jats:italic>Echinococcus granulosus<\/jats:italic>\n                    . Viability of protoscoleces was confirmed by 0.1% aqueous eosin red stain method, where mortality was observed at different concentrations with respect to time. An average anticestodal activity was observed with different endophytic fungal strains, that is,\n                    <jats:italic>Nigrospora<\/jats:italic>\n                    (479 \u00b1 2.9),\n                    <jats:italic>Colletotrichum<\/jats:italic>\n                    (469 \u00b1 25.8),\n                    <jats:italic>Fusarium<\/jats:italic>\n                    (355 \u00b1 14.5), and\n                    <jats:italic>Chaetomium<\/jats:italic>\n                    (332 \u00b1 28.3) showing 64 to 70% protoscolicidal activity, except\n                    <jats:italic>Pestalotiopsis<\/jats:italic>\n                    sp. (581 \u00b1 15.0), which showed promising scolicidal activity up to 97% mortality just within 30\u2009min of incubation. These species showed significant reduction in viability of protoscoleces. This is the first report on the scolicidal activity of endophytic\n                    <jats:italic>Pestalotiopsis<\/jats:italic>\n                    sp. We conclude that ultrastructural changes in protoscoleces were due to endophytic extract suggesting that there may be some bioactive compounds that have selective action on the tegument layer of protoscoleces. As compared with that of standard drug used, endophytic species of Neem plant shows significant anticestodal activity.\n                  <\/jats:p>","DOI":"10.1155\/2013\/308515","type":"journal-article","created":{"date-parts":[[2013,8,26]],"date-time":"2013-08-26T03:01:04Z","timestamp":1377486064000},"page":"1-11","source":"Crossref","is-referenced-by-count":20,"title":["Anticestodal Activity of Endophytic\n                    <i>Pestalotiopsis<\/i>\n                    sp. on Protoscoleces of Hydatid Cyst\n                    <i>Echinococcus granulosus<\/i>"],"prefix":"10.1155","volume":"2013","author":[{"given":"Vijay C.","family":"Verma","sequence":"first","affiliation":[{"name":"Laboratory of Gastrointestinal Infections and Molecular Diagnosis, Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221 005, 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