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The<jats:italic>cag<\/jats:italic>pathogenicity island (<jats:italic>cag<\/jats:italic>-PAI) is one of the most important virulent determinants of<jats:italic>Helicobacter pylori<\/jats:italic>. An insertion sequence (IS) element of<jats:italic>cag<\/jats:italic>-PAI (IS605) has been found to generate<jats:italic>H. pylori<\/jats:italic>strains with varying virulence.<jats:italic>Aim<\/jats:italic>. To evaluate the impact of IS605 and<jats:italic>cag<\/jats:italic>-PAI on<jats:italic>H. pylori<\/jats:italic>strains isolated from Taiwanese patients with severity of gastric diseases.<jats:italic>Methods<\/jats:italic>.<jats:italic>H. pylori<\/jats:italic>isolates were cultured from gastric biopsies from 99 patients with peptic ulcer, chronic gastritis, and gastric carcinoma. Six distinct, well-separated colonies were isolated from each patient and analyzed by genotyping.<jats:italic>Results<\/jats:italic>.<jats:italic>cagA<\/jats:italic>,<jats:italic>cagE<\/jats:italic>,<jats:italic>cagM<\/jats:italic>,<jats:italic>cagT<\/jats:italic>,<jats:italic>orf<\/jats:italic>10, and<jats:italic>orf<\/jats:italic>13 were found to be present in 90.0%\u2013100.0% of the<jats:italic>H. pylori<\/jats:italic>isolates. A total deletion of<jats:italic>cagA<\/jats:italic>,<jats:italic>cagE<\/jats:italic>,<jats:italic>cagM<\/jats:italic>,<jats:italic>cagT<\/jats:italic>,<jats:italic>orf<\/jats:italic>10, and<jats:italic>orf<\/jats:italic>13 was found in 1 isolate (1.0%). The IS605 element was found to be positive in 15.2% of the isolates. The presence of IS605 was higher in<jats:italic>H. pylori<\/jats:italic>isolated from patients with gastric carcinoma (25.0%) than in patients with duodenal ulcer (6.5%) or chronic gastritis (6.3%) (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.001<\/mml:mn><\/mml:mrow><\/mml:math>).<jats:italic>Conclusions<\/jats:italic>. The majority of the patients examined had intact<jats:italic>cag<\/jats:italic>-PAI. IS605 was present in 15.2% and was higher in<jats:italic>H. pylori<\/jats:italic>isolated from patients with gastric carcinoma than in those with peptic ulcer.<\/jats:p>","DOI":"10.1155\/2013\/356217","type":"journal-article","created":{"date-parts":[[2013,2,18]],"date-time":"2013-02-18T14:47:06Z","timestamp":1361198826000},"page":"1-5","source":"Crossref","is-referenced-by-count":17,"title":["Association of IS605 and<i>cag<\/i>-PAI of<i>Helicobacter pylori<\/i>Isolated from Patients with Gastrointestinal Diseases in Taiwan"],"prefix":"10.1155","volume":"2013","author":[{"given":"Chih-Ho","family":"Lai","sequence":"first","affiliation":[{"name":"Department of Microbiology, School of Medicine, China Medical University, Taichung 40402, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin-Lin","family":"Perng","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei 11217, Taiwan"},{"name":"School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keng-Hsin","family":"Lan","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, Taipei 11217, Taiwan"},{"name":"School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hwai-Jeng","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei 11031, Taiwan"},{"name":"School of Medicine, Taipei Medical University, Taipei 11031, 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