{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T01:43:59Z","timestamp":1772847839158,"version":"3.50.1"},"reference-count":22,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2013,6,27]],"date-time":"2013-06-27T00:00:00Z","timestamp":1372291200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ISRN Otolaryngology"],"published-print":{"date-parts":[[2013,6,27]]},"abstract":"<jats:p><jats:italic>Introduction<\/jats:italic>. Previous work has shown a strong association between alterations in cochlear vasculature, aging, and the development of presbycusis. The important role of vascular endothelial growth factor (VEGF) and its receptors Flt-1 and Flk-1 in angiogenesis suggests a potential role for involvement in this process. The aim of this study was to characterize vascular structure and VEGF and its' receptors in young and old C57 Mice. <jats:italic>Methods<\/jats:italic>. Young (4 weeks, <jats:italic>n<\/jats:italic> = 14) and aged (32\u201336 weeks, <jats:italic>n<\/jats:italic> = 14) C57BL\/6 mice were used. Hearing was evaluated using auditory brainstem response. Cochleas were characterized with qRT-PCR, immunohistochemistry, and gross histological quantification. <jats:italic>Results<\/jats:italic>. Old C57 mice demonstrated significantly decreased strial area, blood vessel number, luminal size, and luminal area normalized to strial area (vascularity). qRT-PCR showed a significant upregulation of Flt-1, a VEGF receptor, in older animals. No differences were found in VEGF-A or Flk-1. Immunohistochemistry did not show any differences in staining intensity or area with age or cochlear turn location. <jats:italic>Conclusion<\/jats:italic>. The marked deafness of aged C57 mice could be in part meditated by loss of vascular development and alterations in VEGF signaling.<\/jats:p>","DOI":"10.1155\/2013\/430625","type":"journal-article","created":{"date-parts":[[2013,6,27]],"date-time":"2013-06-27T21:02:22Z","timestamp":1372366942000},"page":"1-6","source":"Crossref","is-referenced-by-count":7,"title":["Changes in the Cochlear Vasculature and Vascular Endothelial Growth Factor and Its Receptors in the Aging C57 Mouse Cochlea"],"prefix":"10.1155","volume":"2013","author":[{"given":"David","family":"Clinkard","sequence":"first","affiliation":[{"name":"Sunnybrook Health Sciences Centre, Otolaryngology\/Head & Neck Surgery, Toronto, Canada M4N 3M5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hosam","family":"Amoodi","sequence":"additional","affiliation":[{"name":"Sunnybrook Health Sciences Centre, Otolaryngology\/Head & Neck Surgery, Toronto, Canada M4N 3M5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thileep","family":"Kandasamy","sequence":"additional","affiliation":[{"name":"Sunnybrook Health Sciences Centre, Otolaryngology\/Head & Neck Surgery, Toronto, Canada M4N 3M5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amandeep S.","family":"Grewal","sequence":"additional","affiliation":[{"name":"Sunnybrook Health Sciences Centre, Otolaryngology\/Head & Neck Surgery, Toronto, Canada M4N 3M5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Chen","sequence":"additional","affiliation":[{"name":"Sunnybrook Health Sciences Centre, Otolaryngology\/Head & Neck Surgery, Toronto, Canada M4N 3M5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Qian","sequence":"additional","affiliation":[{"name":"Sunnybrook Health Sciences Centre, Otolaryngology\/Head & Neck Surgery, Toronto, Canada M4N 3M5"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph M.","family":"Chen","sequence":"additional","affiliation":[{"name":"Sunnybrook Health Sciences Centre, Otolaryngology\/Head & Neck Surgery, Toronto, Canada M4N 3M5"},{"name":"Department of Otolaryngology-Head and Neck Surgery, Toronto, University of Toronto, Canada M5S 1A1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert V.","family":"Harrison","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Toronto, University of Toronto, Canada M5S 1A1"},{"name":"Auditory Science Laboratory, Department of Otolaryngology, Program in Neuroscience and Mental Health, The Hospital for Sick Children, Toronto, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent Y. 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