{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:28:21Z","timestamp":1740169701796,"version":"3.37.3"},"reference-count":18,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81072420","81273246"],"award-info":[{"award-number":["81072420","81273246"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81072420","81273246"],"award-info":[{"award-number":["81072420","81273246"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p><jats:italic>Purpose<\/jats:italic>. To evaluate the effect of a traditional Chinese medicine, Rheum Polysaccharide (RP), on the in vitro production of tumor necrosis factor alpha (TNF-<jats:italic>\u03b1<\/jats:italic>) and interleukin-10 (IL-10) by lipopolysaccharide- (LPS-)stimulated human monocytes from HLA-B27 associated acute anterior uveitis patients of convalescence stage.<jats:italic>Method<\/jats:italic>. PBMC samples were isolated from 10 HLA-B27 associated acute anterior uveitis, incubated, respectively, and divided into 4 groups as follows: (1) controls, PBS was added in final concentration of 1\u2009mg\u00b7L<jats:sup>\u22121<\/jats:sup>, (2) stimulated by LPS, LPS was added in final concentration of 1\u2009mg\u00b7L<jats:sup>\u22121<\/jats:sup>, (3) stimulated by LPS + HTA125, 30 minutes before the adding of LPS in final concentration of 1\u2009mg\u00b7L<jats:sup>\u22121<\/jats:sup>, the final concentration of 5\u2009mg\u00b7L<jats:sup>\u22121<\/jats:sup>of the HTA125 was added, and (4) stimulated by LPS + RP, 30 minutes before the adding of LPS in final concentration 1\u2009mg\u00b7L<jats:sup>\u22121<\/jats:sup>, the final concentration 100\u2009mg\u00b7L<jats:sup>\u22121<\/jats:sup>of the RP was added. Supernatants were used to quantify the amounts of TNF-<jats:italic>\u03b1<\/jats:italic>and IL-10 released in time course using enzyme-linked immunosorbent assay (ELISA).<jats:italic>Result<\/jats:italic>. After stimulated by lps, the concentrations of TNF-<jats:italic>\u03b1<\/jats:italic>and IL-10 in culture supernatants of patients are significantly higher than control group at all time points (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.01<\/mml:mn><\/mml:math>). Blockage of TLR-4 by HTA125 can decrease the production of TNF-<jats:italic>\u03b1<\/jats:italic>and IL-10 compared with lps group (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.01<\/mml:mn><\/mml:math>, except at 4\u2009h group of IL-10). Concentration of TNF-<jats:italic>\u03b1<\/jats:italic>and IL-10 also decreases in the LPS + RP group (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.01<\/mml:mn><\/mml:math>) but not so significantly as in the LPS + HTA125 group.<jats:italic>Conclusion<\/jats:italic>. As anti-TLR4 monoclonal antibodies, rheum Polysaccharide can also inhibit the secretion of cytokines produced by monocytes from HLA-B27 positive AAU patients of convalescence stage.<\/jats:p>","DOI":"10.1155\/2013\/431232","type":"journal-article","created":{"date-parts":[[2013,9,16]],"date-time":"2013-09-16T17:02:36Z","timestamp":1379350956000},"page":"1-4","source":"Crossref","is-referenced-by-count":4,"title":["Role of Rheum Polysaccharide in the Cytokines Produced by Peripheral Blood Monocytes in TLR4 Mediated HLA-B27 Associated AAU"],"prefix":"10.1155","volume":"2013","author":[{"given":"Xuhui","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Baijiazhuang Road Chaoyang District, Beijing 100020, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaofeng","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Baijiazhuang Road Chaoyang District, Beijing 100020, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaosheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Baijiazhuang Road Chaoyang District, Beijing 100020, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongqiu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Baijiazhuang Road Chaoyang District, Beijing 100020, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Baijiazhuang Road Chaoyang District, Beijing 100020, 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