{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,12,17]],"date-time":"2022-12-17T06:55:18Z","timestamp":1671260118369},"reference-count":25,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100007313","name":"MD Anderson Cancer Center","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007313","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>Radiolabeled tyrosine analogs enter cancer cells via upregulated amino acid transporter system and have been shown to be superior to<jats:sup>18<\/jats:sup>F-fluoro-2-deoxy-D-glucose (<jats:sup>18<\/jats:sup>F-FDG) in differential diagnosis in cancers. In this study, we synthesized O-[3-<jats:sup>19<\/jats:sup>F-fluoropropyl]-<jats:italic>\u03b1<\/jats:italic>-methyl tyrosine (<jats:sup>19<\/jats:sup>F-FPAMT) and used manual and automated methods to synthesize O-[3-<jats:sup>18<\/jats:sup>F-fluoropropyl]-<jats:italic>\u03b1<\/jats:italic>-methyl tyrosine (<jats:sup>18<\/jats:sup>F-FPAMT) in three steps: nucleophilic substitution, deprotection of butoxycarbonyl, and deesterification. Manual and automated synthesis methods produced<jats:sup>18<\/jats:sup>F-FPAMT with a radiochemical purity &gt;96%. The decay-corrected yield of<jats:sup>18<\/jats:sup>F-FPAMT by manual synthesis was 34% at end-of-synthesis (88\u2009min). The decay-corrected yield of<jats:sup>18<\/jats:sup>F-FPAMT by automated synthesis was 15% at end-of-synthesis (110\u2009min).<jats:sup>18<\/jats:sup>F-FDG and<jats:sup>18<\/jats:sup>F-FPAMT were used for<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>studies to evaluate the feasibility of<jats:sup>18<\/jats:sup>F-FPAMT for imaging rat mesothelioma (IL-45).<jats:italic>In vitro<\/jats:italic>studies comparing<jats:sup>18<\/jats:sup>F-FPAMT with<jats:sup>18<\/jats:sup>F-FDG revealed that<jats:sup>18<\/jats:sup>F-FDG had higher uptake than that of<jats:sup>18<\/jats:sup>F-FPAMT, and the uptake ratio of<jats:sup>18<\/jats:sup>F-FPAMT reached the plateau after being incubated for 60 min. Biodistribution studies revealed that the accumulation of<jats:sup>18<\/jats:sup>F-FPAMT in the heart, lungs, thyroid, spleen, and brain was significantly lower than that of<jats:sup>18<\/jats:sup>F-FDG. There was poor bone uptake in<jats:sup>18<\/jats:sup>F-FPAMT for up to 3\u2009hrs suggesting its<jats:italic>in vivo<\/jats:italic>stability. The imaging studies showed good visualization of tumors with<jats:sup>18<\/jats:sup>F-FPAMT. Together, these results suggest that<jats:sup>18<\/jats:sup>F-FPAMT can be successfully synthesized and has great potential in mesothelioma imaging.<\/jats:p>","DOI":"10.1155\/2013\/460619","type":"journal-article","created":{"date-parts":[[2013,7,9]],"date-time":"2013-07-09T21:01:21Z","timestamp":1373403681000},"page":"1-9","source":"Crossref","is-referenced-by-count":1,"title":["Synthesis and Biological Evaluation of O-[3-<sup>18<\/sup>F-fluoropropyl]-<b><i>\u03b1<\/i><\/b>-methyl Tyrosine in Mesothelioma-Bearing Rodents"],"prefix":"10.1155","volume":"2013","author":[{"given":"I-Hong","family":"Shih","sequence":"first","affiliation":[{"name":"Department of Experimental Diagnosis Imaging, Unit 59, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan-Lin","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Experimental Diagnosis Imaging, Unit 59, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad S.","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Experimental Diagnosis Imaging, Unit 59, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinhan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Experimental Diagnosis Imaging, Unit 59, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-Fang","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Experimental Diagnosis Imaging, Unit 59, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xudong","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Experimental Diagnosis Imaging, Unit 59, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Experimental Diagnosis Imaging, Unit 59, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"98","reference":[{"issue":"2","key":"1","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1148\/rg.232025705","volume":"23","year":"2003","journal-title":"Radiographics"},{"issue":"6","key":"2","first-page":"945","volume":"46","year":"2005","journal-title":"Journal of Nuclear 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