{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T07:08:16Z","timestamp":1763968096335,"version":"3.37.3"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100006019","name":"Van Andel Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006019","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical and Developmental Immunology"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>Human anthrax infection caused by exposure to<jats:italic>Bacillus anthracis<\/jats:italic>cannot always be treated by antibiotics. This is mostly because of the effect of the remaining anthrax toxin in the body. Lethal factor (LF) is a component of lethal toxin (LeTx), which is the major virulence of anthrax toxin. A murine IgG monoclonal antibody (mAb) against LF with blocking activity (coded LF8) was produced in a previous study. In this report, a human\/murine chimeric Fab mAb (coded LF8-Fab) was developed from LF8 by inserting murine variable regions into human constant regions using antibody engineering to reduce the incompatibility of the murine antibody for human use. The LF8-Fab expressed in<jats:italic>Escherichia coli<\/jats:italic>could specifically identify LF with an affinity of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mn>3.46<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn>10<\/mml:mn><\/mml:mrow><mml:mn>7<\/mml:mn><\/mml:msup><\/mml:mrow><\/mml:math>\u2009L\/mol and could neutralize LeTx with an EC<jats:sub>50<\/jats:sub>of 85\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL. Even after LeTx challenge at various time points, the LF8-Fab demonstrated protection of J774A.1 cells<jats:italic>in vitro<\/jats:italic>. The results suggest that the LF8-Fab might be further characterized and potentially be used for clinical applications against anthrax infection.<\/jats:p>","DOI":"10.1155\/2013\/475809","type":"journal-article","created":{"date-parts":[[2013,6,5]],"date-time":"2013-06-05T23:20:54Z","timestamp":1370474454000},"page":"1-8","source":"Crossref","is-referenced-by-count":7,"title":["A Human\/Murine Chimeric Fab Antibody Neutralizes Anthrax Lethal Toxin<i>In Vitro<\/i>"],"prefix":"10.1155","volume":"2013","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3438-1118","authenticated-orcid":true,"given":"Guipeng","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Pathology, Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ximin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029, 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