{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T12:20:08Z","timestamp":1771935608207,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Platelet function analysis utilizing platelet-rich plasma and optical density based aggregometry fails to identify patients at risk for uremia associated complications.<jats:italic>Methods<\/jats:italic>. We employed whole blood platelet aggregation analysis based on impedance as well as determination of ATP release from platelet granules detected by a chemiluminescence method. Ten chronic kidney disease (CKD) stage 4 or 5 predialysis patients underwent platelet evaluation. Our study aims to evaluate this platform in this patient population to determine if abnormalities could be detected.<jats:italic>Results<\/jats:italic>. Analysis revealed normal aggregation and ATP release to collagen, ADP, and high-dose ristocetin. ATP release had a low response to arachidonic acid (0.37 \u00b1 0.26\u2009nmoles, reference range: 0.6\u20131.4\u2009nmoles). Platelet aggregation to low-dose ristocetin revealed an exaggerated response (20.9 \u00b1 18.7\u2009ohms, reference range: 0\u20135\u2009ohms).<jats:italic>Conclusions<\/jats:italic>. Whole blood platelet analysis detected platelet dysfunction which may be associated with bleeding and thrombotic risks in uremia. Diminished ATP release to arachidonic acid (an aspirin-like defect) in uremic patients may result in platelet associated bleeding. An increased aggregation response to low-dose ristocetin (a type IIb von Willebrand disease-like defect) is associated with thrombus formation. This platelet hyperreactivity may be associated with a thrombotic diathesis as seen in some uremic patients.<\/jats:p>","DOI":"10.1155\/2013\/486290","type":"journal-article","created":{"date-parts":[[2013,6,26]],"date-time":"2013-06-26T21:02:11Z","timestamp":1372280531000},"page":"1-6","source":"Crossref","is-referenced-by-count":19,"title":["Whole Blood Platelet Aggregation and Release Reaction Testing in Uremic Patients"],"prefix":"10.1155","volume":"2013","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7829-5684","authenticated-orcid":true,"given":"Jay","family":"Zeck","sequence":"first","affiliation":[{"name":"Department of Pathology, George Washington University Medical Center, 900 23rd Street NW, Washington, DC 20037, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason","family":"Schallheim","sequence":"additional","affiliation":[{"name":"Department of Pathology, George Washington University Medical Center, 900 23rd Street NW, Washington, DC 20037, 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