{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T01:34:40Z","timestamp":1790127280648,"version":"4.0.1"},"reference-count":19,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Proinflammatory and anti-inflammatory cytokines play a key role in the pathogenesis of periodontal diseases. Secretion of bioactive IL-35 has been described by T regulatory cells () and is required for their maximal suppressive activity. are involved in the modulation of local immune response in chronic periodontitis patients.<jats:italic>Objective<\/jats:italic>. Hence, the present study was aimed to investigate the expression of IL-35 mRNA in chronic periodontitis and aggressive periodontitis patients.<jats:italic>Materials and Methods<\/jats:italic>. The present study was carried out in 60 subjects, which included 20 chronic periodontitis patients, 20 aggressive periodontitis patients, and 20 periodontally healthy controls. IL-35 mRNA expression in gingival tissue samples of all subjects was semiquantitatively analyzed using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR).<jats:italic>Results<\/jats:italic>. The present study demonstrated the expression of IL-35 mRNA in gingival tissues of all the three groups. IL-35 mRNA expression was highest in chronic periodontitis subjects () as compared to the aggressive periodontitis group () and least seen in healthy patients ().<jats:italic>Conclusion<\/jats:italic>. The increased expression of IL-35 in chronic and aggressive periodontitis suggests its possible role in pathogenesis of periodontitis. Future studies done on large samples with intervention will strengthen our result.<\/jats:p>","DOI":"10.1155\/2013\/489648","type":"journal-article","created":{"date-parts":[[2013,11,27]],"date-time":"2013-11-27T18:01:36Z","timestamp":1385575296000},"page":"819-823","source":"Crossref","is-referenced-by-count":20,"title":["Expression Profile of IL-35 mRNA in Gingiva of Chronic Periodontitis and Aggressive Periodontitis Patients: A Semiquantitative RT-PCR Study"],"prefix":"10.1155","volume":"35","author":[{"given":"Nagaraj B.","family":"Kalburgi","sequence":"first","affiliation":[{"name":"Department of Periodontics, PMNM Dental College, Bagalkot, Karnataka 587101, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akshay","family":"Muley","sequence":"additional","affiliation":[{"name":"Department of Periodontics, PMNM Dental College, Bagalkot, Karnataka 587101, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3263-924X","authenticated-orcid":true,"given":"B. 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