{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:10:25Z","timestamp":1762161025409,"version":"3.37.3"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Republic of Korea","doi-asserted-by":"crossref","award":["2011-0030719","2011-0030716","2012R1A1A4A01012975"],"award-info":[{"award-number":["2011-0030719","2011-0030716","2012R1A1A4A01012975"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Republic of Korea","doi-asserted-by":"crossref","award":["2011-0030719","2011-0030716","2012R1A1A4A01012975"],"award-info":[{"award-number":["2011-0030719","2011-0030716","2012R1A1A4A01012975"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002338","name":"Ministry of Education of the People\u2019s Republic of China","doi-asserted-by":"publisher","award":["2011-0030719","2011-0030716","2012R1A1A4A01012975"],"award-info":[{"award-number":["2011-0030719","2011-0030716","2012R1A1A4A01012975"]}],"id":[{"id":"10.13039\/501100002338","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p><jats:italic>Chrysanthemum zawadskii Herbich<\/jats:italic>var.<jats:italic>latilobum Kitamura<\/jats:italic>, known as \u201cGujulcho\u201d in Korea, has been used in traditional medicine to treat various inflammatory diseases, including rheumatoid arthritis. However, these effects have not been tested on osteoclasts, the bone resorbing cells that regulate bone metabolism. Here, we investigated the effects of<jats:italic>C. zawadskii<\/jats:italic>Herbich var.<jats:italic>latilobum<\/jats:italic>Kitamura ethanol extract (CZE) on osteoclast differentiation induced by treatment with the receptor activator of NF-<jats:italic>\u03ba<\/jats:italic>B ligand (RANKL). CZE inhibited osteoclast differentiation and formation in a dose-dependent manner. The inhibitory effect of CZE on osteoclastogenesis was due to the suppression of ERK activation and the ablation of RANKL-stimulated Ca<jats:sup>2+<\/jats:sup>-oscillation via the inactivation of PLC<jats:italic>\u03b3<\/jats:italic>2, followed by the inhibition of CREB activation. These inhibitory effects of CZE resulted in a significant repression of c-Fos expression and a subsequent reduction of NFATc1, a key transcription factor for osteoclast differentiation, fusion, and activation<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>. These results indicate that CZE negatively regulates osteoclast differentiation and may be a therapeutic candidate for the treatment of various bone diseases, such as postmenopausal osteoporosis, rheumatoid arthritis, and periodontitis.<\/jats:p>","DOI":"10.1155\/2013\/509482","type":"journal-article","created":{"date-parts":[[2013,9,23]],"date-time":"2013-09-23T21:15:40Z","timestamp":1379970940000},"page":"1-10","source":"Crossref","is-referenced-by-count":9,"title":["Inhibitory Effect of<i>Chrysanthemum zawadskii<\/i>Herbich var.<i>latilobum<\/i>Kitamura Extract on RANKL-Induced Osteoclast Differentiation"],"prefix":"10.1155","volume":"2013","author":[{"given":"Dong Ryun","family":"Gu","sequence":"first","affiliation":[{"name":"Center for Metabolic Function Regulation (CMFR), Wonkwang University School of Medicine, Iksan 570-749, Republic of Korea"},{"name":"Department of Oral Microbiology and Immunology, College of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Ki","family":"Hwang","sequence":"additional","affiliation":[{"name":"Center for Metabolic Function Regulation (CMFR), Wonkwang University School of Medicine, Iksan 570-749, Republic of Korea"},{"name":"Department of Oral Biochemistry, College of Dentistry, Wonkwang University, 344-2 Shinyong-dong, Iksan 570-749, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Munkhsoyol","family":"Erkhembaatar","sequence":"additional","affiliation":[{"name":"Department of Oral Physiology, College of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kang-Beom","family":"Kwon","sequence":"additional","affiliation":[{"name":"Center for Metabolic Function Regulation (CMFR), Wonkwang University School of Medicine, Iksan 570-749, Republic of Korea"},{"name":"Department of Oriental Medical Physiology, College of Korean Medicine, Wonkwang University, Iksan 570-749, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0071-0830","authenticated-orcid":true,"given":"Min Seuk","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Oral Physiology, College of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young-Rae","family":"Lee","sequence":"additional","affiliation":[{"name":"Center for Metabolic Function Regulation (CMFR), Wonkwang University School of Medicine, Iksan 570-749, Republic of Korea"},{"name":"Department of Oral Biochemistry, College of Dentistry, Wonkwang University, 344-2 Shinyong-dong, Iksan 570-749, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seoung Hoon","family":"Lee","sequence":"additional","affiliation":[{"name":"Center for Metabolic Function Regulation (CMFR), Wonkwang University 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