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To study IgG RFs in patients with other autoimmune diseases, phage display antibody libraries from a hepatitis C virus infected patient with Sj\u00f6gren\u2019s syndrome were constructed. After panning, a specific clone RFL11 was isolated for characterization in advance. The binding activity and specificity of RFL11 to IgG Fc fragment were comparable to those of RFs previously isolated. The analysis with existed RF\u2010Fc complex structures indicated the homology model of RFL11 is similar to IgM RF61 complex with high binding affinity of about 6\u2009 \u00d7 \u200910<jats:sup>\u22128<\/jats:sup>\u2009M. This effect resulted from longer complementarity\u2010determining region (CDR) combining key somatic mutations. In the RFL11\u2010Fc interfaces, the CDR\u2010H3 loop forms a finger\u2010like structure extending into the bottom of Fc pocket and resulting in strong ion and cation\u2010pi interactions. Moreover, a process of antigen\u2010driven maturation was proven by somatically mutated VH residues on H2 and H3 CDR loops in the interfaces. Taken together, these results suggested that high affinity IgG RFs can be generated in patients with Sj\u00f6gren\u2019s syndrome and may play an important role in the pathogenesis of this autoimmune disease.<\/jats:p>","DOI":"10.1155\/2013\/516516","type":"journal-article","created":{"date-parts":[[2013,12,30]],"date-time":"2013-12-30T21:01:19Z","timestamp":1388437279000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Isolation, Characterization, and Molecular Modeling of a Rheumatoid Factor from a Hepatitis C Virus Infected Patient with Sj\u00f6gren\u2019s 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