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Working with the whole salivary homogenate entails serious drawbacks such as the need for maintaining sand fly colonies and the laborious task of glands dissection. In order to overcome these difficulties, producing recombinant proteins of different vectors has become a major task. In this study, a cDNA library was constructed with the salivary glands of<jats:italic>Phlebotomus perniciosus<\/jats:italic>from Madrid, Spain, the most widespread vector of<jats:italic>Leishmania infantum<\/jats:italic>in the Mediterranean basin. Analysis of the cDNA sequences showed several polymorphisms among the previously described salivary transcripts. The apyrase SP01B and the D7-related protein SP04 were successfully cloned, expressed in<jats:italic>Escherichia coli<\/jats:italic>, and purified. Besides, recombinant proteins were recognized by sera of hamsters and mice previously immunized with saliva through the exposure to uninfected sand fly bites. These results suggest that these two recombinant proteins conserved their immunogenic properties after expression in a prokaryote system. Therefore, this work contributes to expand the knowledge of<jats:italic>P. perniciosus<\/jats:italic>saliva that would be eventually used for the development of tools for vector control programs.<\/jats:p>","DOI":"10.1155\/2013\/526069","type":"journal-article","created":{"date-parts":[[2013,9,22]],"date-time":"2013-09-22T21:05:38Z","timestamp":1379883938000},"page":"1-14","source":"Crossref","is-referenced-by-count":11,"title":["Molecular and Immunogenic Properties of Apyrase SP01B and D7-Related SP04 Recombinant Salivary Proteins of<i>Phlebotomus perniciosus<\/i>from Madrid, Spain"],"prefix":"10.1155","volume":"2013","author":[{"given":"In\u00e9s","family":"Mart\u00edn-Mart\u00edn","sequence":"first","affiliation":[{"name":"Unidad de Entomolog\u00eda M\u00e9dica, Servicio de Parasitolog\u00eda, Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Carretera Majadahonda-Pozuelo s\/n, Majadahonda, 28220 Madrid, 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