{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:32:12Z","timestamp":1791408732321,"version":"4.3.3"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,10,8]],"date-time":"2013-10-08T00:00:00Z","timestamp":1381190400000},"content-version":"vor","delay-in-days":280,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Wroc\u0142aw Research Center EIT+","award":["POKL.08.02.01-02-011\/11"],"award-info":[{"award-number":["POKL.08.02.01-02-011\/11"]}]},{"DOI":"10.13039\/501100004895","name":"European Social Fund","doi-asserted-by":"publisher","award":["POKL.08.02.01-02-011\/11"],"award-info":[{"award-number":["POKL.08.02.01-02-011\/11"]}],"id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p>In the course of research and development of a new pharmaceutical formulation of azelaic acid in the liposomal form, we developed a rapid and accurate method for the detection of impurities using high\u2010performance liquid chromatography. A chromatographic column from Merck (Purospher Star RP C18, 250\u20134\u2009mm (5\u2009<jats:italic>\u03bc<\/jats:italic>m) was used in the assay, and the mobile phase gradient consisted of three phases: A\u2014methanol\u2009:\u2009water (5\u2009:\u200995) + 1.5% (v\/v) acetic acid; B\u2014water\u2009:\u2009methanol (5\u2009:\u200995) + 1.5% (v\/v) acetic acid; and C\u2014chloroform. Detection of the impurities and the active substance was performed by an evaporative light\u2010scattering detector. The method was validated for selectivity, system precision, method precision, limit of detection, and response rates. The proposed method can be used to detect impurities in the liposomal formulation of azelaic acid. The method enables separation of azelaic acid from the identified and unidentified impurities and from the excipients used in the drug form.<\/jats:p>","DOI":"10.1155\/2013\/564962","type":"journal-article","created":{"date-parts":[[2013,10,8]],"date-time":"2013-10-08T21:12:15Z","timestamp":1381266735000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Application of HPLC with ELSD Detection for the Assessment of Azelaic Acid Impurities in Liposomal Formulation"],"prefix":"10.1155","volume":"2013","author":[{"given":"Stanislaw","family":"Han","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Karlowicz-Bodalska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7545-0247","authenticated-orcid":false,"given":"Dorota","family":"Szura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukasz","family":"Ozimek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5695-5998","authenticated-orcid":false,"given":"Witold","family":"Musial","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,10,8]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1986.tb02856.x"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1988.tb03474.x"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12317162"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac60063a014"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac60063a015"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac60171a016"},{"key":"e_1_2_8_7_2","unstructured":"SchillingM. R. MichaelR. andKhanjianH. P. Gas chromatographic determination of the fatty acid and glycerol content of lipids: I. The effects of pigments and aging on the composition of oil paints Proceedings of the ICOM Committee for Conservation 11th Triennial Meeting in Edinburgh Scotland 1996 London UK James & James Science 220\u2013227."},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)00344-1"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0026-265X(00)00075-8"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0004-6981(86)90121-6"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac049491m"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-008-0885-y"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp068686q"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/35.5.209"},{"key":"e_1_2_8_15_2","first-page":"39","article-title":"Pre-column fluorescence derivatization using leucine-coumarnylamide for HPLC determination of mono- and dicarboxylic acids in plasma","volume":"83","author":"Levai F.","year":"1995","journal-title":"Acta Physiologica Hungarica"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(72)90202-1"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02492273"},{"key":"e_1_2_8_18_2","first-page":"421","article-title":"Determination of azelaic acid in pharmaceuticals and cosmetics by RP-HPLC after pre-column derivatization","volume":"49","author":"Ferioli V.","year":"1994","journal-title":"Farmaco"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0731-7085(95)01305-5"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2003.09.013"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.09.007"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.12.104"},{"key":"e_1_2_8_23_2","first-page":"398","article-title":"The evaporative light-scattering detection technology","volume":"18","author":"Wei Y.","year":"2000","journal-title":"Se Pu"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/42.9.484"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.02.009"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.1080\/10826079708005545"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/10826079308019586"},{"key":"e_1_2_8_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/alcalc\/agh005"},{"key":"e_1_2_8_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(01)00031-7"},{"key":"e_1_2_8_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf7036104"},{"key":"e_1_2_8_31_2","unstructured":"Q2(R1) CPMP\/ICH\/381\/95 \u201cValidation of Analytical Procedures: Text & Methodology\u201d."},{"key":"e_1_2_8_32_2","volume-title":"Guideline for Submitting Samples and Analytical Data for Methods Validation","author":"United States Food and Drug Administration","year":"1987"},{"key":"e_1_2_8_33_2","volume-title":"Peer Verified Methods Program, Manual on Policies and Procedures","author":"Association of Analytical Communities International","year":"1993"}],"container-title":["The Scientific World Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2013\/564962.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2013\/564962.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2013\/564962","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,6]],"date-time":"2024-07-06T14:49:10Z","timestamp":1720277350000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2013\/564962"}},"subtitle":[],"editor":[{"given":"A.","family":"Concheiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]},{"given":"Y.","family":"Fiamegos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,1]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1155\/2013\/564962"],"URL":"https:\/\/doi.org\/10.1155\/2013\/564962","archive":["Portico"],"relation":{},"ISSN":["2356-6140","1537-744X"],"issn-type":[{"value":"2356-6140","type":"print"},{"value":"1537-744X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1]]},"assertion":[{"value":"2013-07-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-10-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"564962"}}