{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:46:48Z","timestamp":1787017608623,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"University of Limpopo"},{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2013]]},"abstract":"\n The aim of the study was to scientifically evaluate the antimycobacterial activity of selected indigenous medicinal plants from the Limpopo Province used for the treatment of humans with symptoms of\n Mycobacterium tuberculosis<\/jats:italic>\n . The leaves of five plant species (\n Apodytes dimidiata, Artemisia, Combretum hereroense, Lippia javanica, and Zanthoxylum capense<\/jats:italic>\n ) were collected from the Lowveld National Botanical Garden in Nelspruit, South Africa. The dried leaves were powdered and extracted using hexane, dichloromethane, acetone, and methanol. Antimycobacterial activity was evaluated using microdilution assay and bioautography and\n \u03c1<\/jats:italic>\n -iodonitrotetrazolium violet (INT) as indicator. Antioxidant activities were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH). Phytochemical content of extracts was further evaluated. The acetone extracts of\n L. javanica<\/jats:italic>\n displayed antioxidant activity on BEA chromatogram. T Acetone extracts of\n A. afra<\/jats:italic>\n had MIC value of 0.39\u2009mg\/mL against\n Mycobacterium smegmatis<\/jats:italic>\n ATCC 1441. Acetone extracts of\n C. hereroense<\/jats:italic>\n and\n L. javanica<\/jats:italic>\n had MIC value of 0.47\u2009mg\/mL. Four bands that inhibited the growth of\n M. smegmatis<\/jats:italic>\n were observed at\n \n \n \n \n R<\/mml:mi>\n <\/mml:mrow>\n \n f<\/mml:mi>\n <\/mml:mrow>\n <\/mml:msub>\n <\/mml:mrow>\n <\/mml:math>\n values of 0.12, 0.63, and 0.87 on BEA and 0.73 on EMW. The plant species\n A. dimidiata, A. afra, C. hereroense,<\/jats:italic>\n and\n L. javanica<\/jats:italic>\n in this study demonstrated their potential as sources of anti-TB drug leads.\n <\/jats:p>","DOI":"10.1155\/2013\/586247","type":"journal-article","created":{"date-parts":[[2013,7,14]],"date-time":"2013-07-14T17:02:27Z","timestamp":1373821347000},"page":"1-7","source":"Crossref","is-referenced-by-count":27,"title":["Validation of Antimycobacterial Plants Used by Traditional Healers in Three Districts of the Limpopo Province (South Africa)"],"prefix":"10.1155","volume":"2013","author":[{"given":"Peter","family":"Masoko","sequence":"first","affiliation":[{"name":"University of Limpopo, Department of Biochemistry, Microbiology and Biotechnology, Private Bag X1106, Sovenga 0727, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kulani Mashudu","family":"Nxumalo","sequence":"additional","affiliation":[{"name":"University of Limpopo, Department of 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