{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:50:08Z","timestamp":1787017808964,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30670980"],"award-info":[{"award-number":["30670980"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Diabetes Research"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>\n                    <jats:italic>Background<\/jats:italic>\n                    . Hyperglycemia plays a pivotal role in the development of diabetic nephropathy (DN) and may be related to epigenetic metabolic memory. One of the most crucial epigenetic mechanisms is histone modification, which is associated with the expression of a fibrosis factor in vascular injury.\n                    <jats:italic>Aim<\/jats:italic>\n                    .In this study, we investigated the ubiquitination of histones H2A and H2B to explore the epigenetic mechanisms of DN.\n                    <jats:italic>Materials and Methods<\/jats:italic>\n                    . The GMCs were cultured as follows: normal group, high glucose group, mannitol group, and intervention group. After 12\u2009hr, 24\u2009hr, and 48\u2009hr, histones ubiquitination, transforming growth factor-\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                      <mml:mrow>\n                        <mml:mi>\u03b2<\/mml:mi>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    (TGF-\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                      <mml:mrow>\n                        <mml:mi>\u03b2<\/mml:mi>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    ), and fibronectin (FN) were measured using WB, RT-PCR, and IF.\n                    <jats:italic>Result<\/jats:italic>\n                    . High glucose can induce the upregulation of FN. H2A ubiquitination in GMCs increased in high glucose group\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\">\n                      <mml:mo stretchy=\"false\">(<\/mml:mo>\n                      <mml:mi>P<\/mml:mi>\n                      <mml:mo>&lt;<\/mml:mo>\n                      <mml:mn>0.01<\/mml:mn>\n                      <mml:mo stretchy=\"false\">)<\/mml:mo>\n                    <\/mml:math>\n                    , whereas it decreased significantly in intervention group\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\">\n                      <mml:mo stretchy=\"false\">(<\/mml:mo>\n                      <mml:mi>P<\/mml:mi>\n                      <mml:mo>&lt;<\/mml:mo>\n                      <mml:mn>0.05<\/mml:mn>\n                      <mml:mo stretchy=\"false\">)<\/mml:mo>\n                    <\/mml:math>\n                    . In contrast, H2B ubiquitination decreased with an increasing concentration of glucose, but it was recovered in the intervention group\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\">\n                      <mml:mo stretchy=\"false\">(<\/mml:mo>\n                      <mml:mi>P<\/mml:mi>\n                      <mml:mo>&lt;<\/mml:mo>\n                      <mml:mn>0.05<\/mml:mn>\n                      <mml:mo stretchy=\"false\">)<\/mml:mo>\n                    <\/mml:math>\n                    . Expression of TGF-\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\">\n                      <mml:mrow>\n                        <mml:mi>\u03b2<\/mml:mi>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    changed in response to abnormal histone ubiquitination.\n                    <jats:italic>Conclusions.<\/jats:italic>\n                    The high glucose may induce H2A ubiquitination and reduce H2B ubiquitination in GMCs. The changes of histone ubiquitination may be due in part to DN by activating TGF-\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\">\n                      <mml:mrow>\n                        <mml:mi>\u03b2<\/mml:mi>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    signaling pathway.\n                  <\/jats:p>","DOI":"10.1155\/2013\/589474","type":"journal-article","created":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T17:24:12Z","timestamp":1367429052000},"page":"1-10","source":"Crossref","is-referenced-by-count":30,"title":["Impact of High Glucose and Proteasome Inhibitor MG132 on Histone H2A and H2B Ubiquitination in Rat Glomerular Mesangial Cells"],"prefix":"10.1155","volume":"2013","author":[{"given":"Chenlin","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"He","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, 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