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A molecular mass of 14358.69\u2009Da was determined by MALDI-TOF mass spectrometry. Amino acid analysis showed a high content of hydrophobic and basic amino acids as well as 14 half-cysteine residues. The total amino acid sequence was obtained using SwissProt database and showed high amino acid sequence identity with other PLA<jats:sub>2<\/jats:sub>from snake venom. The amino acid composition showed that BrTX-I has a high content of Lys, Tyr, Gly, Pro, and 14 half-Cys residues, typical of a basic PLA<jats:sub>2<\/jats:sub>. BrTX-I presented PLA<jats:sub>2<\/jats:sub>activity and showed a minimum sigmoidal behavior, reaching its maximal activity at pH 8.0, 35\u201345\u00b0C, and required Ca<jats:sup>2+<\/jats:sup>.<jats:italic>In vitro<\/jats:italic>, the whole venom and BrTX-I caused a neuromuscular blockade in biventer cervicis preparations in a similar way to other<jats:italic>Bothrops<\/jats:italic>species. BrTX-I induced myonecrosis and oedema-forming activity analyzed through injection of the purified BrTX-I in mice. Since BrTX-I exerts a strong proinflammatory effect, the enzymatic phospholipid hydrolysis might be relevant for these phenomena; incrementing levels of IL-1, IL-6, and TNF<jats:italic>\u03b1<\/jats:italic>were observed at 15\u2009min, 30\u2009min, one, two, and six hours postinjection, respectively.<\/jats:p>","DOI":"10.1155\/2013\/591470","type":"journal-article","created":{"date-parts":[[2012,12,30]],"date-time":"2012-12-30T21:08:31Z","timestamp":1356901711000},"page":"1-13","source":"Crossref","is-referenced-by-count":5,"title":["Biochemical Characterization and Pharmacological Properties of New Basic PLA<sub><b>2<\/b><\/sub>BrTX-I Isolated from<i>Bothrops roedingeri<\/i>(Roedinger's Lancehead) Mertens, 1942, Snake Venom"],"prefix":"10.1155","volume":"2013","author":[{"given":"Mauricio Aurelio","family":"Gomes Heleno","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Institute of Biology, State University of Campinas (UNICAMP), P.O. 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