{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T14:58:21Z","timestamp":1774969101282,"version":"3.50.1"},"reference-count":60,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>Bbil-TX, a PLA<jats:sub>2<\/jats:sub>, was purified from<jats:italic>Bothriopsis bilineata<\/jats:italic>snake venom after only one chromatographic step using RP-HPLC on<jats:italic>\u03bc<\/jats:italic>-Bondapak C-18 column. A molecular mass of 14243.8\u2009Da was confirmed by Q-Tof Ultima API ESI\/MS (TOF MS mode) mass spectrometry. The partial protein sequence obtained was then submitted to BLASTp, with the search restricted to PLA<jats:sub>2<\/jats:sub>from snakes and shows high identity values when compared to other PLA<jats:sub>2<\/jats:sub>s. PLA<jats:sub>2<\/jats:sub>activity was presented in the presence of a synthetic substrate and showed a minimum sigmoidal behavior, reaching its maximal activity at pH 8.0 and 25\u201337<jats:sup>\u2218<\/jats:sup>C. Maximum PLA<jats:sub>2<\/jats:sub>activity required Ca<jats:sup>2+<\/jats:sup>and in the presence of Cd<jats:sup>2+<\/jats:sup>, Zn<jats:sup>2+<\/jats:sup>, Mn<jats:sup>2+<\/jats:sup>, and Mg<jats:sup>2+<\/jats:sup>it was reduced in the presence or absence of Ca<jats:sup>2+<\/jats:sup>. Crotapotin from<jats:italic>Crotalus durissus cascavella<\/jats:italic>rattlesnake venom and antihemorrhagic factor DA2-II from<jats:italic>Didelphis albiventris<\/jats:italic>opossum sera under optimal conditions significantly inhibit the enzymatic activity. Bbil-TX induces myonecrosis in mice. The fraction does not show a significant cytotoxic activity in myotubes and myoblasts (C2C12). The inflammatory events induced in the serum of mice by Bbil-TX isolated from<jats:italic>Bothriopsis bilineata<\/jats:italic>snake venom were investigated. An increase in vascular permeability and in the levels of TNF-a, IL-6, and IL-1 was was induced. Since Bbil-TX exerts a stronger proinflammatory effect, the phospholipid hydrolysis may be relevant for these phenomena.<\/jats:p>","DOI":"10.1155\/2013\/612649","type":"journal-article","created":{"date-parts":[[2012,12,30]],"date-time":"2012-12-30T21:08:45Z","timestamp":1356901725000},"page":"1-12","source":"Crossref","is-referenced-by-count":21,"title":["Biochemical, Pharmacological, and Structural Characterization of New Basic Bbil-TX from<i>Bothriopsis bilineata<\/i>Snake Venom"],"prefix":"10.1155","volume":"2013","author":[{"given":"Victor","family":"Corasolla Carregari","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Institute of Biology (IB), Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9366-6826","authenticated-orcid":true,"given":"Rafael","family":"Stuani 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