{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T19:53:38Z","timestamp":1789674818898,"version":"4.0.1"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,11,18]],"date-time":"2013-11-18T00:00:00Z","timestamp":1384732800000},"content-version":"vor","delay-in-days":321,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p><jats:italic>Calotropis procera<\/jats:italic> is a medicinal plant whose pharmacological properties are associated with its latex. Here, the <jats:italic>Calotropis procera<\/jats:italic> latex fractions were investigated in an attempt to trace its phytochemical profile and measure its anti\u2010inflammatory and toxicity activity. The crude latex was partitioned, yielding five fractions (49.4% hexane, 5.2% dichloromethane, 2.0% ethyl acetate, 2.1% n\u2010butanol, and 41.1% aqueous). Phytochemical screening and spectroscopy analysis revealed that dichloromethane is the most chemically diverse fraction. Triterpenes were detected in both the hexane and dichloromethane fractions, while flavonoids were detected in the dichloromethane and ethyl acetate fractions. These fractions were cytotoxic to cancer cell lines (LD<jats:sub>50<\/jats:sub> 0.05 to 3.9\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL) and lethal to brine shrimp (LD<jats:sub>50<\/jats:sub> 10.9 to 65.7\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL). Reduced neutrophil migration in rats was observed in carrageenan\u2010induced peritonitis for the dichloromethane (67%), ethyl acetate (56%), and aqueous (72%) fractions. A positive reaction with tolidine and ninhydrin suggested that cyclopeptides are in the ethyl acetate fraction. It is therefore concluded that <jats:italic>Calotropis procera<\/jats:italic> latex dichloromethane and ethyl acetate fractions exhibit both in vitro and in vivo activities as well as anti\u2010inflammatory properties. Cyclopeptide detection is especially interesting because previous attempts to investigate these low\u2010molecular cyclic amino acid sequences in <jats:italic>C. procera<\/jats:italic> have failed.<\/jats:p>","DOI":"10.1155\/2013\/615454","type":"journal-article","created":{"date-parts":[[2013,11,19]],"date-time":"2013-11-19T01:00:34Z","timestamp":1384822834000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Insights on the Phytochemical Profile (Cyclopeptides) and Biological Activities of <i>Calotropis procera<\/i> Latex Organic Fractions"],"prefix":"10.1155","volume":"2013","author":[{"given":"Thiago Lustosa","family":"Juc\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcio Viana","family":"Ramos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederico Bruno Mendes Batista","family":"Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayara Patr\u00edcia","family":"Viana de Matos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Delano Barreto","family":"Marinho-Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato Azevedo","family":"Moreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Cristina de Oliveira","family":"Monteiro-Moreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,11,18]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2011.03.011"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-006-6025-y"},{"key":"e_1_2_10_3_2","first-page":"373","volume-title":"Recent Progress in Medicinal Planta","author":"Kumar V. 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