{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T12:10:19Z","timestamp":1754482219417,"version":"3.40.5"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>A novel spectrophotometric method for the quantification of urinary xanthurenic acid (XA) is described. The direct acid ferric reduction (DAFR) procedure was used to quantify XA after it was purified by a solid-phase extraction column. The linearity of proposed method extends from 2.5 to 100.0\u2009mg\/L. The method is precise, yielding day-to-day CVs for two pooled controls of 3.5% and 4.6%, respectively. Correlation studies with an established HPLC method and a fluorometric procedure showed correlation coefficients of 0.98 and 0.98, respectively. Interference from various urinary metabolites was insignificant. In a small-scale screening of elderly conducted at Penghu county in Taiwan (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>80<\/mml:mn><\/mml:math>), we were able to identify a group of twenty individuals having hyperhomocysteinemia (&gt;15\u2009<jats:italic>\u03bc<\/jats:italic>mole\/L). Three of them were found to be positive for XA as analyzed by the proposed method, which correlated excellently with the results of the activation coefficient method for RBC\u2019s AST\/B<jats:sub>6<\/jats:sub>functional test. These data confirm the usefulness of the proposed method for identifying urinary XA as an indicator of vitamin B<jats:sub>6<\/jats:sub>deficiency-associated hyperhomocysteinemic condition.<\/jats:p>","DOI":"10.1155\/2013\/678476","type":"journal-article","created":{"date-parts":[[2013,9,16]],"date-time":"2013-09-16T21:08:18Z","timestamp":1379365698000},"page":"1-7","source":"Crossref","is-referenced-by-count":3,"title":["Novel Spectrophotometric Method for the Quantitation of Urinary Xanthurenic Acid and Its Application in Identifying Individuals with Hyperhomocysteinemia Associated with Vitamin<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msub><mml:mrow><mml:mtext>B<\/mml:mtext><\/mml:mrow><mml:mrow><mml:mtext>6<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>Deficiency"],"prefix":"10.1155","volume":"2013","author":[{"given":"Chi-Fen","family":"Chen","sequence":"first","affiliation":[{"name":"Clinical Laboratories, Yuan's General 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