{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T06:03:35Z","timestamp":1778997815197,"version":"3.51.4"},"reference-count":21,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>Because cholera toxin (CT) is responsible for most of the symptoms induced by<jats:italic>Vibrio cholerae<\/jats:italic>infection, detection of CT is critical for diagnosis of the disease. In this study, we constructed an immunochromatographic test strip for detection of CT (CT-IC) with polyclonal antibodies developed against purified recombinant whole CT protein. The detection limit of the CT-IC was 10\u2009ng\/mL of purified recombinant CT, and it could detect the CT in culture supernatant of all 15 toxigenic<jats:italic>V. cholerae<\/jats:italic>isolates examined, whereas no false-positive signal was detected in all 5 nontoxigenic<jats:italic>V. cholerae<\/jats:italic>isolates examined. The specificity of the CT-IC was examined with recombinant heat-labile toxin (LT), which shares high homology with CT, and it was revealed that the minimum detection limit for LT was 100 times higher than that for CT. In addition,<jats:italic>lt<\/jats:italic>gene-positive enterotoxigenic<jats:italic>Escherichia coli<\/jats:italic>(ETEC) was examined by CT-IC. The false-positive signals were observed in 3 out of 12 ETEC isolates, but these signals were considerably faint. The CT-IC did not develop false-positive signals with all 7<jats:italic>V. parahaemolyticus<\/jats:italic>isolates. These results showed the high specificity of CT-IC and the feasible use of it for the detection and surveillance of toxigenic<jats:italic>V. cholerae<\/jats:italic>.<\/jats:p>","DOI":"10.1155\/2013\/679038","type":"journal-article","created":{"date-parts":[[2013,11,7]],"date-time":"2013-11-07T21:02:25Z","timestamp":1383858145000},"page":"1-7","source":"Crossref","is-referenced-by-count":14,"title":["Development of an Immunochromatographic Test Strip for Detection of Cholera Toxin"],"prefix":"10.1155","volume":"2013","author":[{"given":"Eiki","family":"Yamasaki","sequence":"first","affiliation":[{"name":"Division of Food Hygiene, Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11 Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuta","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"R&D Center, Nippon Meat Packers, Inc., 3-3 Midorigahara Tsukuba, Ibaraki 300-2646, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"R&D Center, Nippon Meat Packers, Inc., 3-3 Midorigahara Tsukuba, Ibaraki 300-2646, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumiki","family":"Morimatsu","sequence":"additional","affiliation":[{"name":"R&D Center, Nippon Meat Packers, Inc., 3-3 Midorigahara Tsukuba, Ibaraki 300-2646, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Kurazono","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Saitama Institute of Public Health, Saitama 338-0824, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toyoko","family":"Hiroi","sequence":"additional","affiliation":[{"name":"Division of Food Hygiene, Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11 Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. Balakrish","family":"Nair","sequence":"additional","affiliation":[{"name":"Translational Health Science and Technology Institute, Plot no. 496, Phase III, Udyog Vihar, Gurgaon, Haryana 122016, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisao","family":"Kurazono","sequence":"additional","affiliation":[{"name":"Division of Food Hygiene, Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11 Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"98","reference":[{"issue":"31-32","key":"1","first-page":"289","volume":"87","year":"2012","journal-title":"The Weekly Epidemiological Record"},{"issue":"10","key":"2","volume":"6","year":"2012","journal-title":"PLOS Neglected Tropical Diseases"},{"key":"3","year":"1999"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-94"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-008-1876-4"},{"issue":"13","key":"6","doi-asserted-by":"crossref","first-page":"5596","DOI":"10.1021\/ac300567f","volume":"84","year":"2012","journal-title":"Analytical Chemistry"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.7345"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1994.1007"},{"issue":"5","key":"9","doi-asserted-by":"crossref","first-page":"748","DOI":"10.4315\/0362-028X.JFP-12-423","volume":"76","year":"2013","journal-title":"Journal of Food Protection"},{"issue":"1","key":"10","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1111\/j.1348-0421.1992.tb01641.x","volume":"36","year":"1992","journal-title":"Microbiology and Immunology"},{"issue":"12","key":"11","doi-asserted-by":"crossref","first-page":"2314","DOI":"10.1128\/JCM.25.12.2314-2316.1987","volume":"25","year":"1987","journal-title":"Journal of Clinical Microbiology"},{"issue":"3","key":"12","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1128\/JCM.22.3.405-408.1985","volume":"22","year":"1985","journal-title":"Journal of Clinical Microbiology"},{"issue":"11","key":"13","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1111\/j.1348-0421.1986.tb03037.x","volume":"30","year":"1986","journal-title":"Microbiology and Immunology"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.5.1355-1361.2004"},{"issue":"7","key":"15","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1128\/JCM.30.7.1783-1786.1992","volume":"30","year":"1992","journal-title":"Journal of Clinical Microbiology"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.5.1821-1827.2006"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.4265\/bio.16.159"},{"key":"18","year":"1999"},{"issue":"6","key":"19","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/S0092-8674(00)81551-2","volume":"99","year":"1999","journal-title":"Cell"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.111581598"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00409-09"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/679038.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/679038.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/679038.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T14:32:06Z","timestamp":1596810726000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2013\/679038\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":21,"alternative-id":["679038","679038"],"URL":"https:\/\/doi.org\/10.1155\/2013\/679038","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013]]}}}